Analysis of Somatic Cell Mutants That Express Defective Intracellular Cholesterol Transport

  • Laura Liscum
Chapter

Abstract

The distribution of cholesterol in mammalian cells is tightly controlled. However, the factors involved in shuttling cholesterol among cellular membranes have not been well defined. Our experimental approach employs somatic cell mutants and pharmacological agents to investigate the routes and mechanisms of intracellular cholesterol transport. Two complementation groups of Chinese hamster ovary cell mutants have been isolated, with gene defects that impair separate cholesterol transport pathways. Identification of genes that correct the cholesterol transport defects is in progress. Pharmacological inhibitor studies have revealed a pathway that lipoprotein-derived cholesterol takes to reach the endoplasmic reticulum. The mechanism of that transport pathway is under investigation. In coming years, our studies should turn from biochemical description of transport pathways to molecular analysis of candidate transport proteins.

Key Words

cholesterol intracellular transport somatic cell mutant Niemann-Pick type C 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Laura Liscum
    • 1
  1. 1.Department of PhysiologyTufts University School of MedicineBostonUSA

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