Intestinal Microvascular PO2 Measurement with Pd-Porphyrin Phosphorescence in the Mechanically Ventilated Mouse
Investigations into the determinants of oxygen transport to tissue in mice can be expected to provide new insights into the molecular mechanisms underlying tissue oxygenation. This approach has two main advantages: it enables the use of genetically modified mice in which, depending on the objective of study, specific molecular mechanisms can be modified and it enables access to a multitude of commercially available kits for assessment of inflammatory mediators specifically developed for the mouse. In order to study mechanisms related to cardiovascular function, physiological measurement techniques will have to be developed specifically for the mouse to enable measurement of parameters providing information about macroscopic hemodynamics as well as the oxygenation of the microcirculation. Because of its sensitivity to dysoxia and its inflammatory potential we first focused on the gut. In this study we present results from hemodynamic measurements (arterial blood pressure and blood flow in aorta) in combination with microvascular pO2 measurement in the gut of anesthetized and mechanically ventilated mice undergoing hemorrhagic shock and resuscitation.
KeywordsArterial Blood Pressure Hemorrhagic Shock Oxygen Transport Transonic Flow Crystalloid Solution
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