Demonstration of Differences in Drug Resistance by Direct Testing of DNA Excision Repair Activity Following Standard and Liposomal Daunorubicin Exposure in Normal Paediatric Marrow Using High Resolution CLSM

  • Christopher L. Lannon
  • Lynne M. Ball
  • Allen F. Pyesmany
  • Margaret Yhap
  • G. Ross Langley
  • Dick van Velzen
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 457)

Abstract

High resolution Confocal Laser Scanning Microscopy (CLSM) may be applied to testing of drug resistance in vitro in clinical setting. Rapid analysis of DNA damage by precise quantitation of excised DNA in bone marrow samples exposed to potential treatment moieties directly after isolation but the relative sensitivity of the integrated method is as yet untested.

Keywords

DNA excision repair liposomal Daunorubicin drug resistance confocal laser scanning microscopy single cell gel electrophoresis 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Christopher L. Lannon
    • 1
  • Lynne M. Ball
    • 1
  • Allen F. Pyesmany
    • 1
  • Margaret Yhap
    • 1
  • G. Ross Langley
    • 1
  • Dick van Velzen
    • 1
  1. 1.Department of PathologyDalhousie University, IWK Grace Health CentreHalifaxCanada

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