Tryptophan, Serotonin, and Melatonin pp 637-644

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 467)

Latest on Enzymology of Serotonin Biosynthesis in Walnut Seeds

  • Peter Schröder
  • Claudia Abele
  • Petra Gohr
  • Uta Stuhlfauth-Roisch
  • Wolfgang Grosse

Abstract

Serotonin (5-HT) accumulation in walnut cotyledons is seen as a detoxification mechanism protecting the sensitive plant tissues of seeds from highly toxic ammonia concentrations following seed desiccation. Different metabolic pathways and cell compartments are involved in biosynthesis and storage of 5-HT. Ammonia fixation and incorporation into the indole moiety of tryptophan is followed by 5-HT biosynthesis via tryptamine in a two-step pathway with the adaptive tryptophan decarboxylase and the constitutive tryptamine 5-hydroxylase. Evidence is provided that tryptamine 5-hydroxylase is a member of the cytochrome P450 family which is involved in lipid hydroxylation processes in the very early period of seed development.

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References

  1. Aebi, H., 1974, Katalase. in: Methoden der enzymatischen Analyse I. (H. Bergmeyer, ed.), pp. 636–648, Verlag Chemie, Weinheim.Google Scholar
  2. Boll, M. and Kardinal, A., 1990, Hydroxy-3-methylglutaryl coenzyme A reductase from spruce (Picea abies). Properties and regulation, Z. Naturforsch. 45c:973–979.Google Scholar
  3. Bomhoff, G.H. and Spencer, M., 1977, Optimum pH and ionic strength for the assay of cytochrome c oxidase from pea cotyledon mitochondria, Can. J. Biochem. 55:114–116.Google Scholar
  4. Bradford, M.M., 1976, A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analyt. Biochem. 72:248–254.PubMedCrossRefGoogle Scholar
  5. Grosse, W. and Artigas, F., 1983, Incorporation of 15N-ammonia into serotonin in cotyledons of maturing walnuts, Z. Naturforsch. 38c:1057–1058.Google Scholar
  6. Grosse, W. and Schmidt, U., 1980, Ammonia detoxification by formation of serotonin in seeds of Juglans regia L. I. Accumulation of serotonin in protein bodies, Ber. Deutsch. Bot. Ges. 93:575–585.Google Scholar
  7. Grosse, W., 1977, Ammonia detoxification and tryptophan synthesis in leucoplasts of walnuts, Z. Pflanzen-physiol. 83:249–255.Google Scholar
  8. Grosse, W., 1982, Function of serotonin in seeds of walnuts. Phytochemistry 21:819–822.CrossRefGoogle Scholar
  9. Grosse, W., Karisch, M., and Schröder, P., 1983, Serotonin biosynthesis and its regulation in seeds of Juglans regia L, Z. Pflanzenphysiol. 110:221–229.Google Scholar
  10. Schröder, P. and Beiford, E.J., 1996, Untersuchungen zur Aktivität von Glutathion S-Transferasen in Nadeln von Fichten im Schulterberg-und Christlumprofil, FBVA-Berichte 94:75–82.Google Scholar
  11. Schröder, P. and Stampfl, A., 1998, Visualization of glutathione conjugation and inducibility of glutathione S-transferases in onion (Allium cepa L.) epidermal tissue, Xenobiotica, submitted.Google Scholar
  12. Smith, T.A., 1976, Review: Tryptamine and related compounds in plants. Phytochemistry 16:171–175.CrossRefGoogle Scholar
  13. Tolbert, N.E., 1974, Isolation of subcellular organells of metabolism on isopycnic sucrose gradients, in: Methods in Enzymology 31, Biomembranes Part C (Fleischer, S. and Packer, L., eds.), pp. 734–746, Acad. Press, New York.CrossRefGoogle Scholar
  14. Yonetani, T., 1967, Cytochrome c oxidase: Beef heart, in: Methods in Enzymology 10, Biomembranes (Colowick, S.P. and Kaplan, N.O., eds.), pp. 734–746, Acad. Press, New York.Google Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Peter Schröder
    • 1
  • Claudia Abele
    • 2
  • Petra Gohr
    • 2
  • Uta Stuhlfauth-Roisch
    • 2
  • Wolfgang Grosse
    • 2
  1. 1.GSF National Research Center for Environment and HealthNeuherbergGermany
  2. 2.Botanical InstituteUniversity of CologneCologneGermany

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