Kynurenine Pathway Metabolism in Human Astrocytes

  • Gilles J. Guillemin
  • Stephen J. Kerr
  • George A. Smythe
  • Patricia J. Armati
  • Bruce J. Brew
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 467)

Abstract

The involvement of astrocytes in Kynurenine pathway (KP) metabolism is still poorly understood. In the present study, we investigated the ability of human fetal astrocytes in vitro to produce quinolinic and picolinic acids using mass spectrometry. In parallel, we estimated the level of expression of five major KP enzymes using RT-PCR. The results demonstrated that astrocytes express most KP enzymes, except for kynurenine-hydroxylase. This in vitro study provides novel informations regarding the ability of human fetal astrocytes to degrade L-tryptophan along the KP.

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Gilles J. Guillemin
    • 1
  • Stephen J. Kerr
    • 1
  • George A. Smythe
    • 3
  • Patricia J. Armati
    • 4
  • Bruce J. Brew
    • 1
    • 2
  1. 1.Centre for ImmunologyAustralia
  2. 2.Departments of Neurology and HIV MedicineSt Vincent’s HospitalSydneyAustralia
  3. 3.Biomedical Mass Spectrometry UnitUniversity of New South WalesSydneyAustralia
  4. 4.School of Biological SciencesUniversity of SydneyAustralia

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