Preparing Mammary Gland Whole Mounts from Mice

  • Susan B. Rasmussen
  • Lawrence J. T. Young
  • Gilbert H. Smith


We present methods, fixation schedules, and stains for preparing whole mounts of murine mammary glands. We have included references to papers published 60 years ago as well as those in current journals. The use of this method has undergone a renaissance with the advent of transgenic and knock-in—knockout genetic mouse models relevant to the study of mammary growth, development, and neoplasia.


Methylene Blue Mammary Gland Mouse Mammary Gland Carminic Acid Hematoxylin Solution 
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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Susan B. Rasmussen
    • 1
  • Lawrence J. T. Young
    • 2
  • Gilbert H. Smith
    • 1
  1. 1.Laboratory of Tumor Immunology and Biology, National Cancer InstituteNational Institutes of HealthBethesdaUSA
  2. 2.Center for Comparative MedicineUniversity of California-DavisDavisUSA

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