Photobiology pp 183-192 | Cite as

Ultraviolet Mutagenesis of a Shuttle Vector Plasmid in Repair Proficient and Deficient Human Cells

  • M. M. Seidman
  • D. Brash
  • S. Settharam
  • K. H. Kraemer
  • A. Bredberg
Chapter

Abstract

Studies of mutagenesis in prokaryotic systems have been performed with great sophistication for many years. There is an enormous body of literature which describes the chemistry, biochemistry, genetics, etc. of mutagenesis for a wide variety of mutagenic agents. In mammalian cells, however, it has been quite difficult to answer even the most fundamental questions about the nature of mutations and the mechanisms of mutagenesis. This is, of course, due to the complexity of the mammalian genome and the attendant difficulty of generating the specific DNA sequence data necessary for an initial consideration of these questions. The recombinant DNA revolution has spawned a variety of approaches to these problems such that answers to at least the most basic questions are available. In this paper we will discuss the development of one of these systems, the transient shuttle vector.

Keywords

Shuttle Vector Mutant Plasmid Cyclobutane Dimer Replication Apparatus Xeroderma Pigmentosum Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • M. M. Seidman
    • 1
    • 2
  • D. Brash
    • 1
    • 2
  • S. Settharam
    • 1
    • 2
  • K. H. Kraemer
    • 1
    • 2
  • A. Bredberg
    • 1
    • 2
  1. 1.Otsuka Pharmaceutical Co. Ltd.RockvilleUSA
  2. 2.Laboratory of Molecular CarcinogenesisNational Cancer InstituteBethesdaUSA

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