Advertisement

Effects of Various Growth Factors on a Chondrocyte Differentiation Model

  • Fujio Suzuki
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 324)

Abstract

We developed a new 4-dimensional differentiation model of growth-cartilage cells (temporally and spatially regulated). composed of a high-density suspension culture of rabbit costal chondrocytes. In this system, fibroblast growth factor (FGF) or transforming growth factor-β (TGFβ) stimulated proliferation and matrix synthesis, but reversibly inhibited terminal differentiation - induction of alkaline phosphatase (Alpase) activity, type X collagen synthesis and subsequent calcification. When parathyroid hormone (PTH) was added to the differentiation model, both the induction of Alpase and calcium uptake were reversibly suppressed. In contrast, calcitonin (CT) stimulated Alpase activity and calcium uptake dose-dependently. Bone morphogenetic protein (BMP) strongly stimulated DNA synthesis of chondrocytes in the presence of FGF and induced rapid maturation of chondrocytes at a growing stage. Moreover, BMP stimulated Alpase activity in multilayered chondrocytes at the calcifying stage.

Keywords

Bone Morphogenetic Protein Uronic Acid Endochondral Ossification Demineralized Bone Matrix Growth Cartilage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Reddi A.H., Regulation of bone differentiation by local and systemic factors, in: “Bone and Mineral Research.” Vol. 3, pp 27–47, Peck W.A., ed., Elsevier, Amsterdam (1985).Google Scholar
  2. 2.
    Shimomura Y., Yoneda T. and Suzuki F., Osteogenesis by chondrocytes from growth cartilage of rat rib, Calcif. Tissue Res., 19:179–187 (1976).CrossRefGoogle Scholar
  3. 3.
    Suzuki F., Yoneda T. and Shimomura Y, Calcitonin and parathyroidhormone stimulation of acid mucopolysaccharide synthesis in cultured chondrocytes isolated from growth cartilage, FEBS Lett 70:155–158 (1976).PubMedCrossRefGoogle Scholar
  4. 4.
    Suzuki F. and Takase T., Simulation of the initial state of endochondral ossification: In vitro sequential culture of growth cartilage cells and bone marrow cells. Proc. Natl. Acad. Sci. USA 78:2368–2372 (1981).PubMedCrossRefGoogle Scholar
  5. 5.
    Kato Y., Iwamoto M., Koike T., Suzuki F. and Takano Y., Terminal differentiation and calcification in rabbit chondrocyte cultures grown in centrifuge tubes: Regulation by transforming growth factorβ and serum factors, Proc. Natl. Acad. Sci. USA 85:9552–9556 (1988).PubMedCrossRefGoogle Scholar
  6. 6.
    Kato Y. and Iwamoto M., Fibroblast growth factor is an inhibitor of chondrocyte terminal differentiation, J. Biol. Chem. 265:5903–5909 (1990).PubMedGoogle Scholar
  7. 7.
    Wozney J.M., Rosen V., Celeste A.J., Mitsock L.M., Whitters M.J., Kriz R.W., Hewick R.M., and Wang E.A., Novel regulators of bone formation: Molecular clones and activities, Science 242:1528–1534 (1988).PubMedCrossRefGoogle Scholar
  8. 8.
    Lobb R., Sasse J., Shing Y., D’Amore P., Sullivan R., Jacobs J. and Klagsbrun M., Purification and characterization of heparin-binding endothelial cell growth factors, J. Biol. Chem. 261:1924–1928 (1986).PubMedGoogle Scholar
  9. 9.
    Kato Y., Nomura Y., Tsuji M., Kinoshita M., Ohmae H. and Suzuki F., Somatomedin-like peptide(s) isolated from fetal bovine cartilage (cartilage-derived factor): Isolation and some properties, Proc. Natl. Acad. Sci. USA 78, 6831–6835 (1981).PubMedCrossRefGoogle Scholar
  10. 10.
    Hiraki Y., Tanaka H., Inoue H., Kondo J., Kamizono A. and Suzuki F. Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-1, which stimulates growth of cultured chondrocytes, Biochem. Biophys. Res. Commun. in press (1991).Google Scholar

Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Fujio Suzuki
    • 1
  1. 1.Department of Biochemistry Faculty of DentistryOsaka UniversitySuita OsakaJapan

Personalised recommendations