Expression and localization of intestinal 15 kDa protein in the rat

  • Shoichi Iseki
  • Osamu Amano
  • Tatsuo Kanda
  • Hiroshi Fujii
  • Teruo Ono
Chapter
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 10)

Abstract

Rat intestinal 15 kDa protein (I-15P) is highly homologous to porcine gastrotropin. We studied the occurrence, distribution and subcellular localization of I-15P in the entire rat body, using the immunocytochemistry to localize protein and in situ hybridization to localize mRNA. Both techniques demonstrated the expression of I-15P in the enterocytes of ileum, luteal cells of ovary and a subpopulation of steroid-endocrine cells of adrenal gland. Immuno-electron microscopy further demonstrated that I-15P is localized in both the cytoplasmic and nuclear matrix regions of these cells. The present results suggest roles of I-15P not only in the transport of bile salts but also in the metabolisms of certain steroid hormones.

Key words

fatty acid-binding protein immunohistochemistry in situ hybridization ileum ovary adrenal gland 
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Copyright information

© Springer Science+Business Media Dordrecht 1993

Authors and Affiliations

  • Shoichi Iseki
    • 1
  • Osamu Amano
    • 1
  • Tatsuo Kanda
    • 2
  • Hiroshi Fujii
    • 2
  • Teruo Ono
    • 2
  1. 1.Department of Anatomy, School of MedicineKanazawa UniversityKanazawa 920Japan
  2. 2.Department of BiochemistryNiigata University School of MedicineNiigata 951Japan

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