Arenavirus Ultrastructure and Morphogenesis

  • Richard W. Compans
Part of the The Viruses book series (VIRS)


Although lymphocytic choriomeningitis (LCM) virus was identified as an infectious agent during the early decades of virology research (Armstrong and Lillie, 1934; Traub, 1935), no information on its structure or classification was obtained until many years had elapsed. The development of cell culture systems for virus growth and improved preparative procedures for electron microscopy enabled the initial identification of characteristic LCMV particles in thin sections of virus-infected cells (Dalton et al., 1968). Soon thereafter, other viruses belonging to the Tacaribe complex were found to have a similar morphology (Murphy et al.,1970), leading to the classification of these agents in a new virus family (Rowe et al.,1970). Subsequently, some additional information has been obtained on the structure of the internal components of arena-viruses. This chapter reviews our current knowledge of the virion structure and the process of viral morphogenesis for this family of viruses.


Envelop Virus Virus Assembly Lymphocytic Choriomeningitis Nucleoprotein Complex Lymphocytic Choriomeningitis Virus 
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  1. Abelson, H. T., Smith, G. H., Hoffman, H. A., and Rowe, W. P., 1969, Use of enzyme-labelled antibody for electron microscope localization of lymphocytic choriomeningitis virus antigens in infected cell cultures, J. Natl. Cancer Inst. 42:497.PubMedGoogle Scholar
  2. Armstrong, C., and Lillie, R. D., 1934, Experimental lymphocytic choriomeningitis of monkeys and mice produced by a virus encountered in studies of the 1933 St. Louis encephalitis epidemic, Public Health Rep. 49:1019.CrossRefGoogle Scholar
  3. Basak, S., and Compans, R. W., 1983, Studies on the role of glycosylation in the functions and antigenic properties of influenza virus glycoproteins, Virology 128:77.PubMedCrossRefGoogle Scholar
  4. Bishop, D. H. L., 1990, Arenaviridae and their replication, in: Virology (B. N. Fields and D. M. Knipe, eds.), pp. 1231–1243, Raven Press, New York.Google Scholar
  5. Bishop, D. H. L., and Auperin, D. D., 1987, Arenavirus gene structure and organization, Curr. Top. Microbiol. Immunol. 133:5.PubMedCrossRefGoogle Scholar
  6. Boersma, D. P., and Compans, R. W., 1985, Synthesis of Tacaribe virus polypeptides in an in vitro coupled transcription and translation system, Virus Res. 2:271.CrossRefGoogle Scholar
  7. Bruns, M., and Lehmann-Grube F., 1983, Lymphocytic choriomeningitis virus. V. Pro-posed structural arrangement of proteins in the virion J. Gen. Virol. 64:2157–2167.PubMedCrossRefGoogle Scholar
  8. Bruns, M., Peralta, L. M., and Lehmann-Grube, F., 1983, Lymphocytic choriomeningitis virus. III. Structural proteins of the virion J. Gen. Virol. 64:599.PubMedCrossRefGoogle Scholar
  9. Bruns, M., Zeller, W., Rohdewohld, H., and Lehmann-Grube, F., 1986, Lymphocytic choriomeningitis virus. IX. Properties of the nucleocapsid, Virology 151:77.PubMedCrossRefGoogle Scholar
  10. Buchmeier, M. J., and Oldstone, M. B. A., 1979, Protein structure of lymphocytic choriomeningitis virus: Evidence for a cell-associated precursor of the virion glycopeptides, Virology 99:111.PubMedCrossRefGoogle Scholar
  11. Buchmeier, M. J., and Parekh, B. S., 1987, Protein structure and expression among arena-viruses, Curr. Top. Microbiol. Immunol. 133:41.CrossRefGoogle Scholar
  12. Buchmeier, M. J., Elder, J. H., and Oldstone, M. B. A., 1978, Protein structure of lymphocytic choriomeningitis virus: identification of the virus structural and cell associated polypeptides, Virology 89:133.PubMedCrossRefGoogle Scholar
  13. Buchmeier, M. J., Lewicki, H. A., Tomori, O., and Oldstone, M. B. A., 1981, Monoclonal antibodies to lymphocytic choriomeningitis and Pichinde viruses: Generation, characterization, and cross-reactivity with other arenaviruses, Virology 113:73.PubMedCrossRefGoogle Scholar
  14. Burns, J. W., and Buchmeier, M. J., 1991, Protein-protein interactions in lymphocytic choriomeningitis virus, Virology 183:620.PubMedCrossRefGoogle Scholar
  15. Burns, J. W., Salvato, M. S., and Buchmeier, M. J., 1990, Molecular architecture of lympho-cytic choriomeningitis virus, Proc. VIllth Intl. Cong. Virol., Berlin, Abstr. W3–001.Google Scholar
  16. Chinault, D. N., Thompson, H. A., and Gangemi, J. D., 1981, Polypeptide synthesis cata-lysed by components of Pichinde virus disrupted by detergent, J. Gen. Virol. 55:213.PubMedCrossRefGoogle Scholar
  17. Compans, R. W., and Bishop, D. H. L., 1985, Biochemistry of arenaviruses, Curr. Top. Microbiol. Immunol. 114:153.CrossRefGoogle Scholar
  18. Dalton, A. J.,Rowe, W. P., Smith, G. H., Wilsnack, R. E., and Pugh, W. E., 1968, Morphological and cytochemical studies on lymphocytic choriomeningitis virus, J. Virol. 2:1465.PubMedGoogle Scholar
  19. Farber, F. E., and Rawls, W. E., 1975, Isolation of ribosome-like structures from Pichinde virus, J. Gen. Virol. 26:21.PubMedCrossRefGoogle Scholar
  20. Gard, G. P., Vezza, A. C., Bishop, D. H. L., and Compans, R. W., 1977, Structural proteins of Tacaribe and Tamiami virions, Virology 83:84.CrossRefGoogle Scholar
  21. Gibson, R., Schlesinger, S., and Kornfeld, S., 1979, The non-glycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures, J. Biol. Chem. 254:3600.PubMedGoogle Scholar
  22. Iapalucci, S, Lopez, N., Rey, O., Zakin, M., Cohen, G. N., and Franze-Fernandez, M. T., 1989, The 5’ region of Tacaribe virus L RNA encodes a protein with a potential metal binding domain, Virology 173:357.PubMedCrossRefGoogle Scholar
  23. Lee, L. T., Howe, C., Meyer, K., and Choi, H. U., 1969, Quantitative precipitin analysis of influenza virus host antigen and of sulfated polysaccharides of chicken embryonic allantoic fluid, J. Immunol. 102:1144.PubMedGoogle Scholar
  24. Leung, W-C., and Rawls, W. E., 1977, Virion-associated ribosomes are not required for the replication of Pichinde virus, Virology 81:176–176.CrossRefGoogle Scholar
  25. Leung, W C., Ghosh, H. P., and Rawls, W. E., 1977, Strandedness of Pichinde virus RNA, J. Virol. 22:235.PubMedGoogle Scholar
  26. McSharry, J. J., Compans, R. W., and Choppin, P. W., 1971, Proteins of vesicular stomatitis virus and of phenotypically mixed VSV-SV5 virions, J. Virol. 8:722.Google Scholar
  27. Muller, G., Bruns, M., Martinez Peralta, L., and Lehmann-Grube, F., 1983, Lymphocytic choriomeningitis virus IV. Electron microscopic investigation of the virion, Arch. Virol. 75:229.PubMedCrossRefGoogle Scholar
  28. Murphy, F. A., and Whitfield, S. G., 1975, Morphology and morphogenesis of arenaviruses, Bull WHO 52:409.PubMedGoogle Scholar
  29. Murphy, F. A., Webb, P. A., Johnson, K. M., Whitfield, S. G., and Chappell, W. A., 1970, Arenaviruses in Vero cells: Ultrastructural studiesJ. Virol. 6:507.PubMedGoogle Scholar
  30. Nayak, D. P., and Jabbar, M. A., 1989, Structural domains and organizational conformation involved in the sorting and transport of influenza virus transmembrane proteins, Ann. Rev. Microbiol. 43:465.CrossRefGoogle Scholar
  31. Ofodile, A., Padnos, M., Molomut, N., and Duffy, J. L., 1973, Morphological and biological characteristics of the M-P strain of lymphocytic choriomeningitis virus, Infect.Immun 7.309.PubMedGoogle Scholar
  32. Oldstone, M. B. A., 1987, Arenaviruses: Biology and immunotherapy, Curr. Top. Microbiol. Immunol. 134:1.CrossRefGoogle Scholar
  33. Padula, P. J., and de Martinez Segovia, Z., 1984, Replication of Junin virus in the presence of tunicamycin, Intervirology 22:227.PubMedCrossRefGoogle Scholar
  34. Palmer, E. L., Obijeski, J. F., Webb, P. A., and Johnson, K. M., 1977, The circular, seg-mented nucleocapsid of an arenavirus-Tacaribe virus, J. Gen. Virol. 36:541.PubMedCrossRefGoogle Scholar
  35. Pedersen, I. R., and Konigshofer, E. P., 1976, Characterization of ribonucleoproteins and ribosomes isolated from lymphocytic choriomeningitis virus,J. Virol. 20:14.PubMedGoogle Scholar
  36. Ramos, B. A., Courtney, R. J., and Rawls, W. E., 1972, Structural proteins of Pichinde virus, J. Virol 10:661.PubMedGoogle Scholar
  37. Rowe, W. P., Murphy, F. A., Bergold, G. H., Casals, J., Hotchin, J., Johnson, K. M., Lehmann-Grube, F., Mims, C. A., Traub, E., and Webb, P. A., 1970, Arenaviruses: Proposed name for a newly defined virus group, J. Virol. 5:651.PubMedGoogle Scholar
  38. Salvato, M. S., and Shimomaye, E. S., 1989, The completed sequence of lymphocytic choriomeningitis virus reveals a unique RNA structure and a gene for a zinc-finger protein, Virology 173:1.PubMedCrossRefGoogle Scholar
  39. Salvato, M. S., Schweighofer, K. J., Burns, J. W., and Shimomaye, E. S., 1992, Biochemical and immunological evidence that the 11 kDa zinc-binding protein of lymphocytic choriomeningitis virus is a structural component of the virus, Virus Res. (in press).Google Scholar
  40. Sengupta, S., and Rawls, W. E., 1979, Pseudotypes of vesicular stomatitis virus and pichinde virus, J. Gen. Virol. 42:141.PubMedCrossRefGoogle Scholar
  41. Sivasubramanian, N., and Nayak, D. P., 1987, Mutational analysis of the signal-anchor domain of influenza virus neuraminidase, Proc. Natl. Acad. Sci. USA 84:105.CrossRefGoogle Scholar
  42. Stephens, E. B., and Compans, R. W., 1988, Assembly of animal viruses at cellular mem-branes, Annu. Rev. Microbiol. 42:489.PubMedCrossRefGoogle Scholar
  43. Sturman, L. S., 1977, Characterization of a coronavirus. I. Structural proteins: Effects of preparative conditions on the migration of protein in polyacrylamide gels, Virology 77:637.PubMedCrossRefGoogle Scholar
  44. Traub, E., 1935, A filterable virus recovered from white mice, Science 81:298.PubMedCrossRefGoogle Scholar
  45. Vezza, A. C., and Bishop, D. H. L., 1977, Recombination between temperature sensitive mutants of the arenavirus Pichinde, J. Virol. 24:712.PubMedGoogle Scholar
  46. Vezza, A. C., Gard, G. P., Compans, R. W., and Bishop, D. H. L., 1977, Structural components of the arenavirus Pichinde, J. Virol. 23:776.PubMedGoogle Scholar
  47. Vezza, A. C., Clewley, J. P., Gard, G. P., Abraham, N. Z., Compans, R. W., and Bishop, D. H. L., 1978, Virion RNA species of the arenaviruses Pichinde, Tacaribe, and Tamiami, J. Virol. 26:485.PubMedGoogle Scholar
  48. von Bonsdorff, C-H., and Patterson, R., 1975, Surface structure of Uukeniemi virus, J. Virol. 16:1296.Google Scholar
  49. Wright, K. E., Salvato, M. S., and Buchmeier, M. J., 1989, Neutralizing epitopes of lymphocytic choriomeningitis virus are conformational and require both glycosylation and disulfide bonds for expression, Virology 171:417.PubMedCrossRefGoogle Scholar
  50. Young, P. R., and Howard, C. R., 1983, Fine structure analysis of Pichinde virus nucleocapsids, J. Gen. Virol. 64:833.PubMedCrossRefGoogle Scholar
  51. Zeller, W., Bruns, M., and Lehmann-Grube, F., 1988, Lymphocytic choriomeningitis virus. X. Demonstration of nucleoprotein on the surface of infected cells, Virology 162:90.PubMedCrossRefGoogle Scholar

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© Springer Science+Business Media New York 1993

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  • Richard W. Compans

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