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Coronaviruses pp 401-406 | Cite as

Recombinant Vaccinia Viruses Which Express MHV-JHM Proteins: Protective Immune Response and the Influence of Vaccination on Coronavirus-Induced Encephalomyelitis

  • Egbert Flory
  • Albert Stühler
  • Hanna Wege
  • Stuart Siddell
  • Helmut Wege
Chapter
  • 384 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)

Summary

Vaccinia-virus (VV) recombinants encoding either the nucleocapsid (N) or the spike (S) protein of MHV-JHM were constructed to study the role of the immune reponse against defined coronavirus antigens. For the S-protein, a fusogenic (Sfus+) or non fusogenic variant (Sfus-) of the gene was inserted into the VV genome. A strong protection against acute encephalomyelitis (AE) was mediated in Lewis rats which were immunized by VV-Sfus+ and challenged with an otherwise lethal dose of MHV-JHM before the induction of S-specific IgG antibodies. By contrast, a VV recombinant encoding a variant non fusogenic S-protein or the N-protein was not capable confering protection. In addition, we demonstrated that MHV-JHM S-specific IgG antibodies elicited before MHV-JHM challenge modulated the disease process, changing it from an acute disease to subacute demyelinating encephalomyelitis (SDE).

Keywords

Acute Disease Specific Immune Response Protective Immune Response Plaque Reduction Assay Antiviral Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Egbert Flory
    • 1
  • Albert Stühler
    • 1
  • Hanna Wege
    • 1
  • Stuart Siddell
    • 1
  • Helmut Wege
    • 1
  1. 1.Institute of Virology and ImmunobiologyWürzburgGermany

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