Coronaviruses pp 377-384 | Cite as

Role of Macrophage Procoagulant Activity in Mouse Hepatitis Virus (MHV) Infection: Studies Using T Cell MHV-3 Clones and Monoclonal Antibody 3D4.3

  • S. Chung
  • C. Li
  • L. S. Fung
  • A. Crow
  • R. Gorczynski
  • E. Cole
  • S. Perlman
  • J. Leibowitz
  • G. Levy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


Murine hepatitis virus strain 3 (MHV) infection produces a strain dependent spectrum of disease1,2. Mice of the A and SJL strain are fully resistant to the effects of viral infection, whereas mice of semisusceptible strains (C3H/HeJ) develop acute hepatitis which progesses to varying degrees of chronic hepatitis. Mice of fully susceptible strains (Balb/cJ, C57BL/6J) die of fulminant hepatic failure. The resistance of the A strain mice cannot be explained by a lack of a cellular receptor for MHV as viral binding occurs on cells from these resistant mice3,4. Furthermore, restriction of viral replication does not explain resistance, as resistance occurs despite the presence of active viral replication in the resistant A strain. Bang and Warwick previously reported that differences in viral replication in cultures of macrophages reflects the relative susceptibility/resistance (S/R) to viral infections5. However, several laboratories have shown that MHV replicates in cultures of macrophages, endothelial cells and hepatocytes from both susceptible and resistant animals4,6–8although viral replication occurs to a lesser degree in cells derived from resistant animals6,9. Thus, absolute differences in viral replication do not account for the strain dependent S/R pattern seen in MHV infection.


Viral Replication Fulminant Hepatic Failure Hepatic Sinusoid Susceptible Mouse Murine Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Levy, G., Leibowitz, J., Edgington, T. J. Exp. Med. 154,1150, 1981.PubMedCrossRefGoogle Scholar
  2. 2.
    Virelizier, J., Allison, A. Arch. ViroL. 150,279, 1976.CrossRefGoogle Scholar
  3. 3.
    Boyle, J., Weismiller, D., Holmes, K. J. Virol. 61,185, 1987.PubMedGoogle Scholar
  4. 4.
    Holmes, K., Williams, R., Stephenson, C et al. Cell Biology of Virus Entry Replica-tion and Pathogenesis . R. Compans, A. Melinius, M. Oldstone., eds Alan Liss, New York, 1989, 85.Google Scholar
  5. 5.
    Bang, F., Warwick, A. Proc. Nat. Acad. Sci. USA. 46,1065, 1960.PubMedCrossRefGoogle Scholar
  6. 6.
    Dindzans, V., MacPhee, P., Fung, L. et al. J. Immunol. 135,4189, 1985.PubMedGoogle Scholar
  7. 7.
    Pereira, C., Steffan, A., Kirn, A. J. Gen. ViroL 65,1617, 1984.PubMedCrossRefGoogle Scholar
  8. 8.
    Macnaughton, M., Patterson, S. Arch. Virol. 66,71, 1980.PubMedCrossRefGoogle Scholar
  9. 9.
    Sinclair, S., Abecassis, M., Wong, P.Y. et al. Adv. Exp. Med. BioL 276,533 1990.PubMedCrossRefGoogle Scholar
  10. 10.
    Dindzans, V., Skamene, E., Levy, G. J. Immunol. 137,2355, 1986.PubMedGoogle Scholar
  11. 11.
    Chung, S., Sinclair, S., Leibowitz, J. et al. J. Immunol. 146,271, 1991.PubMedGoogle Scholar
  12. 12.
    Levy, G., MacPhee, P., Fung, L. et al. Hepatology. 3,964, 1983.PubMedCrossRefGoogle Scholar
  13. 13.
    MacPhee, P., Dindzans, V., Fung, L., Levy, G. Hepatology. 5,649, 1985.PubMedCrossRefGoogle Scholar
  14. 14.
    Fung, L., Neil, G., Leibowitz, J. et al. J. Biol. Chem. 266,1789, 1991.PubMedGoogle Scholar
  15. 15.
    Li, C., Fung, L.S., Crow, A., Myers-Mason, N., Leibowitz, J., Cole, E., Levy, G. J. Exp. Med. 1763, 1992.Google Scholar
  16. 16.
    Dindzans, V., Zimmerman, B., Sherker, A., Levy, G. Coronoviruses 1987. M. Lai, S. Stohlman eds. Plenum Publishing Corp. New York 1987, 411.Google Scholar
  17. 17.
    Mosmann, T., Cherwinski, H., Bond, M. et al. J. Immunol. 136,2348, 1986.PubMedGoogle Scholar
  18. 18.
    Mosmann, T., Coffman, R. Immunol. Today. 223, 1987.Google Scholar
  19. 19.
    Chung, S., Nisbet-Brown, E., Gorczynski, R., Skamene, E., Perlman, S., Leibowitz, J., Fung,L.S., Crow,A., Myers-Mason,N., Flowers,M., Levy,G. J. Immu-nol. Submitted, 1992.Google Scholar
  20. 20.
    Korner, H., Schleiphake, A., Winter, J., et al. J. Immunol. 2317, 1991.Google Scholar
  21. 21.
    Scott, P., Natovitz, P., Coffman, R., et al. J. Exp. Med. 169,59, 1988.Google Scholar
  22. 22.
    Fidel, P., Boros, D. J. Immunol. 146,1941, 1991.PubMedGoogle Scholar
  23. 23.
    Malefyt, R., Haanen, J., Spits, H.,et al. J. Exp. Med. 174,915, 1991.CrossRefGoogle Scholar
  24. 24.
    Caulada-Benedetta, Z., Al-Zamel, F., Sher, A., James, S. J. Immunol. 146,1655, 1991.Google Scholar
  25. 25.
    Scott, P., Kaufmann, S. ImmunoL Today. 12,346. 1991.PubMedCrossRefGoogle Scholar
  26. 26.
    Locksley, R., Scott, P. Immunol. Today. 12,A58, 1991.PubMedCrossRefGoogle Scholar
  27. 27.
    Scott, P., Natovitz, P., Coffman, R.,et al. J. Exp. Med. 169,59,1988 . Google Scholar
  28. 28.
    Virelizier, J., Gresser, I. J. Immunol 120,1616, 1978.PubMedGoogle Scholar
  29. 29.
    Fiorentino, D., Zlotnik, A., Vieira, P.,et al. J. Immunol. 146,3444, 1991.PubMedGoogle Scholar
  30. 30.
    Swain, S., Weinberg, A., English, M., Huston, G. J. Immunol 145,3796, 1990.PubMedGoogle Scholar
  31. 31.
    Lamontagne, L., Jolicoeur, P. Immunol 146,3152, 1991.Google Scholar
  32. 32.
    Woodward, J., Matsushima, G., Frelinger, Stohlman, S. J. Immunol. 133,1016, 1984.PubMedGoogle Scholar
  33. 33.
    Stohlman, S., Matsushima, G., Asteel, N., Weiner, L. J. Immunol. 136,3052, 1984.Google Scholar
  34. 34.
    Morrissey, J., Fair, D., Edgington, T. Thromb. Res. 52,247, 1988.PubMedCrossRefGoogle Scholar
  35. 35.
    Smith, A., Cardellichio, C., Winograd, M. et al. J. Infect. Dis. 163,879, 1991.PubMedCrossRefGoogle Scholar
  36. 36.
    Sturman, L., Ricard, C., Holmes, K. J. Virol. 56,904, 1985.PubMedGoogle Scholar
  37. 37.
    Frana, M., Behnke, J., Sturman, L., Holmes, K. J. Virol 56,912, 1985.PubMedGoogle Scholar
  38. 38.
    Abecassis, M., Falk, J., Makowka, L. et al. J. Clin. Invest. 80,881, 1987.PubMedCrossRefGoogle Scholar
  39. 39.
    Taylor, F., Chang, A., Ruff, W. et al. Circ. Shock 33,127, 1991.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • S. Chung
    • 1
  • C. Li
    • 2
  • L. S. Fung
    • 1
  • A. Crow
    • 2
  • R. Gorczynski
    • 1
  • E. Cole
    • 2
  • S. Perlman
    • 3
  • J. Leibowitz
    • 4
  • G. Levy
    • 2
  1. 1.Departments of SurgeryUniversity of TorontoCanada
  2. 2.Departments of MedicineUniversity of TorontoCanada
  3. 3.The University of IowaUSA
  4. 4.University of TexasUSA

Personalised recommendations