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Coronaviruses pp 279-284 | Cite as

Dissemination of MHV4 (Strain JHM) Infection Does Not Require Specific Coronavirus Receptors

  • T. M. Gallagher
  • M. J. Buchmeier
  • S. Perlman
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)

Abstract

In this report, we demonstrate the syncytial spread of MHV4 (strain JHM) infection through non-murine cell cultures which lack a specific MHV4 receptor and are therefore resistant to infection by free virions. This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay. Receptor-independent syncytium formation induced by cells expressing the MHV4 spike (S) from recombinant vaccinia viruses (VV) indicated that spread was mediated by this coronavirus glycoprotein. We conclude that the S protein of MHV4 is so potently fusogenic that it does not require prior binding to a virus-specific surface receptor to induce fusion of closely-opposed plasma membranes.

Keywords

Syncytium Formation Recombinant Vaccinia Virus Scripps Research Institute Intracerebral Inoculation RK13 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • T. M. Gallagher
    • 1
  • M. J. Buchmeier
    • 1
  • S. Perlman
    • 2
  1. 1.Division of Virology, Department of NeuropharmacologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Departments of Pediatrics and MicrobiologyUniversity of IowaIowa CityUSA

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