Coronaviruses pp 279-284 | Cite as

Dissemination of MHV4 (Strain JHM) Infection Does Not Require Specific Coronavirus Receptors

  • T. M. Gallagher
  • M. J. Buchmeier
  • S. Perlman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


In this report, we demonstrate the syncytial spread of MHV4 (strain JHM) infection through non-murine cell cultures which lack a specific MHV4 receptor and are therefore resistant to infection by free virions. This was achieved by allowing infected murine cells to settle onto confluent monolayers of non-murine cells in a straightforward infectious center assay. Receptor-independent syncytium formation induced by cells expressing the MHV4 spike (S) from recombinant vaccinia viruses (VV) indicated that spread was mediated by this coronavirus glycoprotein. We conclude that the S protein of MHV4 is so potently fusogenic that it does not require prior binding to a virus-specific surface receptor to induce fusion of closely-opposed plasma membranes.


Syncytium Formation Recombinant Vaccinia Virus Scripps Research Institute Intracerebral Inoculation RK13 Cell 
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • T. M. Gallagher
    • 1
  • M. J. Buchmeier
    • 1
  • S. Perlman
    • 2
  1. 1.Division of Virology, Department of NeuropharmacologyThe Scripps Research InstituteLa JollaUSA
  2. 2.Departments of Pediatrics and MicrobiologyUniversity of IowaIowa CityUSA

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