Coronaviruses pp 267-272 | Cite as
Expression of MHV-A59 Receptor Glycoproteins in Susceptible and Resistant Strains of Mice
Abstract
Band and Warwick showed that inbred mouse strains differed in their susceptibility to virulent strain of mouse hepatitis virus, MHV-2, and that peritoneal macrophages cultured from these mouse strains reflected their differences in susceptibility to the virus (1). Adult SJL/J mice are highly resistant to MHV-JHM and MHV-A59 (2,12,13). Our laboratory showed that membranes from the liver and intestinal epithelial cells of BALB/c mice could bind MHV-A59 virions in a solid phase assay using undenatured membrane proteins, whereas membranes from SJL/J mice did not bind virus, suggesting that differences in binding of virus to cells from different mouse strains could account for their differences in susceptibility to MHV (3). In virus-overlay protein blot assays of intestinal brush border and liver membrane proteins, MHV-A59 virions bound strongly to a 58 kDa glycoprotein from BALB/c mice, but the virus did not recognize any proteins from SJL/J mouse tissues (3,4). Anti-receptor monoclonal antibody MAb-CC1 that blocked infection of murine cell lines with MHV-A59 recognized the 110–120 kDa and 58 kDa membrane glycoproteins of BALB/c mice, but no proteins from SJL/J membranes (6,7).
Keywords
Inbred Mouse Strain Mouse Hepatitis Virus Intestinal Brush Border Solid Phase Assay Fourth DomainPreview
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