Cloning and Sequence Analysis of the Spike Gene from Several Feline Coronaviruses

  • A. Paul Reed
  • Sharon Klepfer
  • Timothy Miller
  • Elaine Jones
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


The DNA sequence encoding the spike gene from the DF2 strain of Type II feline infectious peritonitis virus (FIPV), a temperature sensitive FIPV virus (TS-DF2) and an isolate of feline enteric coronavirus (FECV 1683) were determined. Comparison of the published WSU 1146 and DF2 FIPV S genes showed that the viruses shared a high degree of homology (99.6%). Likewise, the S gene of the virulent DF2 FIPV virus was closely conserved to that isolated from the vaccine virus strain, TS-DF2 FIPV. In contrast, the FECV S gene had numerous DNA and amino acid differences when compared to the virulent FIPV sequences. Sequence differences among the feline coronavirus isolates were localized to the amino-terminus region of the S gene.


Infectious Bronchitis Virus Amino Acid Difference Spike Gene Amino Acid Homology Guanidine Isothiocyanate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    N.C. Pedersen and J.F. Boyle, Am. J. Vet. Res. 41:868–876 (1980).Google Scholar
  2. 2.
    H. Lutz, B. Hauser, and M.C. Horzinek, J. Small Animal Pract. 27:108 (1986).CrossRefGoogle Scholar
  3. 3.
    N.C. Pedersen, Adv. Exp. Med. Biol. 218:529–550 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    R.D. Woods and N.C. Pedersen, Vet. Microbiol. 4:11–16 (1979).CrossRefGoogle Scholar
  5. 5.
    N.C. Pedersen and J.W. Black, Am. J. Vet. Res. 44:229–234 (1983).PubMedGoogle Scholar
  6. 6.
    C.A. Stoddart, J.E. Barlough, C.A. Baldwin and F.W. Scott, Res. Vet. Sci. 45:383–388 (1988).PubMedGoogle Scholar
  7. 7.
    J.E. Barlough. C.A. Stoddart, G.P. Jacobson and F.W. Scott, Lab. Anim. Sci. 34:592–597 (1984).PubMedGoogle Scholar
  8. 8.
    H. Vennema, R.J. De Groot, D.A. Harbour, M. Dalderup, T. Gruffyd-Jones, M.C. Horzinek and W.J.M. Spaan, J. Virol. 64:1407–1409 (1990).PubMedGoogle Scholar
  9. 9.
    H. Vennema, R.J. De Groot, D.A. Harbour, M.C. Horzinek and W.J.M. Spaan, Virology 181:327–335 (1991).PubMedCrossRefGoogle Scholar
  10. 10.
    J.D. Gerber, J.D. Ingersoll, A.M. Gast, K.K. Christianson, N.L. Selzer, R.M. Landon, N.E. Pfeiffer, R.L. Sharpee and W.H. Beckenhauer, Vaccine 8:536–542 (1990).PubMedCrossRefGoogle Scholar
  11. 11.
    K.K. Christianson, J.D. Ingersoll, R.M. Landon, N.E. Pfeiffer and J.D. Gerber, Arch. Virol. 109:185–196 (1989).PubMedCrossRefGoogle Scholar
  12. 12.
    R.J. De Groot, J. Maduro, J.A. Lenstra, M.C. Horzinek, B.A.M. Van Der Zeijst and W.J.M. Spaan, J. Gen. Virol. 68:2639–2646 (1987).PubMedCrossRefGoogle Scholar
  13. 13.
    J.M. Chirgwin, A.E. Przybyla, R.J. MacDonald and W.J. Rutter, Biochemistry 18:5294–5301 (1979).PubMedCrossRefGoogle Scholar
  14. 14.
    E.S. Kawasaki. Amplification of RNA, in “PCR Protocols. A Guide to Methods and Applications,” M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White, eds. Academic Press, San Diego, CA (1990).Google Scholar
  15. 15.
    R.K. Saiki, S. Scharf, F.A. Faloona, K.B. Mullis, G.T. Horn, H.A. Erlich and N. Arnheim, Science 230:1350–1354 (1985).PubMedCrossRefGoogle Scholar
  16. 16.
    F. Sanger, S. Nicklen and A.R. Coulson, Proc. Natl. Acad. Sci. USA 74:5463–5467 (1977).PubMedCrossRefGoogle Scholar
  17. 17.
    J. Devereux, P. Haberlie and O. Smithies, Nucleic Acids Research 12:387–395 (1984).PubMedCrossRefGoogle Scholar
  18. 18.
    L. Jacobs, R.J. De Groot, M.C. Horzinek, B.A.M. Van der Zeijst and W.J.M. Spaan, (FIPV), Virus Res. 8:363–3712 (1987).PubMedCrossRefGoogle Scholar
  19. 19.
    W. Luytjes, L.S. Sturman, P.J. Bredenbeek, J. Charite, B.A.M. Van Der Zeijst, M.C. Horzinek and W.J.M. Spaan, Virology 161:479–487 (1987).PubMedCrossRefGoogle Scholar
  20. 21.
    J.G. Kusters, H.G.M. Niesters, J.A. Lenstra, M.C. Horzinek, and B.A.M. Van Der Zeijst, Virology 169:217–221 (1989).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • A. Paul Reed
    • 1
  • Sharon Klepfer
    • 1
  • Timothy Miller
    • 1
  • Elaine Jones
    • 1
  1. 1.Department of Molecular BiologySmithKline Beecham Animal HealthUSA

Personalised recommendations