Coronaviruses pp 137-142 | Cite as

Site-Specific Sequence Repair of Coronavirus Defective Interfering RNA by RNA Recombination and Edited RNA

  • Young-Nam Kim
  • Michael M. C. Lai
  • Shinji Makino
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


Mouse hepatitis virus (MHV), a coronavirus, contains an approximately 31 kb-long genomic RNA (8, 9). In MHV-infected cells, seven to eight species of virus-specific subgenomic mRNAs with a 3’-coterminal nested-set structure (6, 10) are synthesized; these are numbered 1 to 7, in decreasing order of size (6, 10). The 5’-end of MHV genomic RNA contains a 72- to 77-nucleotide-long leader sequence (5, 7, 22). An identical sequence is found at the 5’-end of each MHV mRNA species; in each, the leader sequence is fused with the mRNA body sequence, which starts from a consensus sequence at the intergenic sites (17, 21).


Leader Sequence Nucleotide Deletion Helper Virus Virus Sample Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Young-Nam Kim
    • 1
  • Michael M. C. Lai
    • 2
  • Shinji Makino
    • 1
  1. 1.Department of MicrobiologyThe University of Texas at AustinAustinUSA
  2. 2.Howard Hughes Medical Institute and Department of MicrobiologyUniversity of Southern California, School of MedicineLos AngelesUSA

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