Northern Blot Analysis of Sepiapterin Reductase mRNA in Mammalian Cell Lines and Tissues
Recent evidence has shown that H4biopterin is synthesized in cells which undergo cytokine-directed proliferation and do not use H4biopterin as a hydroxylation cofactor. H4biopterin, in turn, enhances the proliferation of erythroleukemic cells and modulates various aspects of the interleukin-2-induced clonal expansion of T cells.1 In these cells the activity of sepiapterin reductase (SR) is in the range of 100 pmol min-1 mg-1.Lectin Stimulation of resting T cells causes a slowly progressing increase, starting from levels below detection and a transient enhancement of SR activity is found after treatment of activated T cells by interferon-γ plus interleukin-2.1 The NK-like human cell line YT and the murine erythroleukemic cell line B8/3 lack any SR activity. The molecular basis of SR regulation has not been explained in any of these cases.
KeywordsNorthern Blot Analysis mRNA Species Liver Cell Line Steady State mRNA Level Murine Erythroleukemia Cell
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