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Northern Blot Analysis of Sepiapterin Reductase mRNA in Mammalian Cell Lines and Tissues

  • Josef Maier
  • Karin Schott
  • Thomas Werner
  • Adelbert Bacher
  • Irmgard Ziegler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 338)

Abstract

Recent evidence has shown that H4biopterin is synthesized in cells which undergo cytokine-directed proliferation and do not use H4biopterin as a hydroxylation cofactor. H4biopterin, in turn, enhances the proliferation of erythroleukemic cells and modulates various aspects of the interleukin-2-induced clonal expansion of T cells.1 In these cells the activity of sepiapterin reductase (SR) is in the range of 100 pmol min-1 mg-1.Lectin Stimulation of resting T cells causes a slowly progressing increase, starting from levels below detection and a transient enhancement of SR activity is found after treatment of activated T cells by interferon-γ plus interleukin-2.1 The NK-like human cell line YT and the murine erythroleukemic cell line B8/3 lack any SR activity. The molecular basis of SR regulation has not been explained in any of these cases.

Keywords

Northern Blot Analysis mRNA Species Liver Cell Line Steady State mRNA Level Murine Erythroleukemia Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Josef Maier
    • 1
  • Karin Schott
    • 1
  • Thomas Werner
    • 2
  • Adelbert Bacher
    • 3
  • Irmgard Ziegler
    • 1
  1. 1.Institut für Klinische Molekularbiologie und TumorgenetikGSF-Gesellschaft für Umwelt-und GesundheitsforschungMünchenGermany
  2. 2.Institut für SäugetiergenetikGSF-Gesellschaft für Umwelt-und GesundheitsforschungNeuherbergGermany
  3. 3.Lehrstuhl für Organische Chemie und BiochemieTechnische Universität MünchenGarchingGermany

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