Positive and Negative Regulation of Cell Cycle Progression by Serine/Threonine Protein Phosphatases

  • Arthur S. Alberts
  • Axel Schönthal
Chapter
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

Reversible protein phosphorylation events play a key role in intracellular signal transduction pathways that regulate gene expression and cell proliferation. To analyze the involvement of protein phosphatases in these processes, we applied two different approaches. First, we used okadaic acid, a specific inhibitor of the two major serine/threonine protein phosphatases, type 1 (PP-1) and type 2A (PP-2A).1–3 Second, we microinjected subunits of PP-2A into living cells and analyzed the effects on gene expression.

Keywords

Protein Phosphatase Catalytic Subunit Regulatory Subunit Okadaic Acid Intracellular Signal Transduction Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Arthur S. Alberts
    • 1
  • Axel Schönthal
    • 2
  1. 1.Departments of Pharmacology and MedicineUniversity of California at San DiegoLa JollaUSA
  2. 2.Department of MicrobiologyUniversity of Southern CaliforniaLos AngelesUSA

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