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Functional Site Determinations in Three Dimensions on Eukaryotic and Eubacterial Ribosomes

  • Adriana Verschoor
  • Suman Srivastava
  • Michael Radermacher
  • Joachim Frank
  • Robert R. Traut
  • Marina Stöffler-Meilicke
  • Dohn Glitz

Abstract

Immuno electron microscopy has been a powerful tool in efforts to map exposures of individual r-proteins and functional sites on the ribosome, in that it has provided direct visual identification of the contact between an antibody and its epitope, as seen in an electron micrograph of a single-particle preparation of ribosomes. However, the information has been only two-dimensional: the site is seen on a projection image of the particle. If it can be identified on several different projections -- representing different orientations of the ribosome -- some three-dimensional (3D) information can be deduced, but the localization can be only approximate until 3D reconstruction techniques are applied to the problem.

Keywords

Functional Site Immuno Electron Microscopy Tilt Series Ribosomal Particle Native Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Adriana Verschoor
    • 1
  • Suman Srivastava
    • 1
  • Michael Radermacher
    • 1
  • Joachim Frank
    • 1
  • Robert R. Traut
    • 2
  • Marina Stöffler-Meilicke
    • 3
  • Dohn Glitz
    • 4
  1. 1.New York State Dept. of HealthWadsworth Center for Laboratories and ResearchAlbanyUSA
  2. 2.Dept. of Biological ChemistrySchool of Medicine, University of CaliforniaDavisUSA
  3. 3.Institut für Klinische und Experimentelle VirologieFreie Universität BerlinBerlinGermany
  4. 4.Dept. of Biological Chemistry, UCLA School of MedicineUniversity of CaliforniaLos AngelesUSA

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