Functional Site Determinations in Three Dimensions on Eukaryotic and Eubacterial Ribosomes
Immuno electron microscopy has been a powerful tool in efforts to map exposures of individual r-proteins and functional sites on the ribosome, in that it has provided direct visual identification of the contact between an antibody and its epitope, as seen in an electron micrograph of a single-particle preparation of ribosomes. However, the information has been only two-dimensional: the site is seen on a projection image of the particle. If it can be identified on several different projections -- representing different orientations of the ribosome -- some three-dimensional (3D) information can be deduced, but the localization can be only approximate until 3D reconstruction techniques are applied to the problem.
KeywordsFunctional Site Immuno Electron Microscopy Tilt Series Ribosomal Particle Native Subunit
Unable to display preview. Download preview PDF.
- Hoppe, W., Gaßmann, J., Hunsmann, N., Schramm, H.J., and Sturm, M., 1974, Hoppe-Seyler’s Z. Phys. Chem. 355:1483Google Scholar
- Radermacher, M., Wagenknecht, T., Verschoor, A., and Frank, J., 1987b, EMBO J. 6:1107Google Scholar
- Radermacher, M., Srivastava, S., and Frank, J., 1992, Proc. 10th Eur. Congr. El. Micr. Vol. III, 19–20Google Scholar
- Srivastava, S., Radermacher, M., and Frank, J, 1992a, Proc. 10th Eur. Congr. El. Micr. Vol. I, 421–422Google Scholar