Mutants of tRNA, Ribosomes and mRNA Affecting Frameshifting, Hopping or Stop Codon Read-Through
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Programmed non-standard translation elongation events, frameshifting, hopping and read-through, are important in the decoding of a minority of mRNAs from diverse sources. In this chapter we provide an overview of some of the approaches we are taking to investigate this “recoding” (Gesteland et al., 1992). Also included is an overview of some current studies on mutants which cause frameshifting and read-through, at sites where it does not normally occur i.e., non-programmed events.
KeywordsMurine Leukemia Virus Infectious Bronchitis Virus Mouse Mammary Tumor Virus Anticodon Loop Mature tRNA
- Dayhuff, T.J., Gesteland, R.F., and Atkins, J.F., 1992, Electrophoresis, autoradiography and electroblotting of peptides: T4 gene 60 hopping, Bio Techniques 13:499.Google Scholar