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Characterization of Structural Proteins of Lelystad Virus

  • Janneke J. M. Meulenberg
  • Annelien Petersen-den Besten
  • Eric P. de Kluyver
  • Rob J. M. Moormann
  • Wim M. M. Schaaper
  • Gert Wensvoort
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames that encode putative viral proteins. Synthetic polypeptides of 15 to 17 amino acids were selected from the amino acid sequences of ORFs 2 to 7 and anti-peptide sera were raised in rabbits. Using these anti-peptide sera and porcine anti-LV serum, we identified three structural proteins and assigned their corresponding genes. Virions were found to contain a nucleocapsid protein of 15 kDa (N), an unglycosylated membrane protein of 18 kDa (M), and a glycosylated membrane protein of 25 kDa (E). The N protein is encoded by ORF7, the M protein is encoded by ORF6, and the E protein is encoded by ORF5.

Keywords

Infectious Bronchitis Virus Nucleocapsid Protein Replication Strategy CL2621 Cell Equine Arteritis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Janneke J. M. Meulenberg
    • 1
  • Annelien Petersen-den Besten
    • 1
  • Eric P. de Kluyver
    • 1
  • Rob J. M. Moormann
    • 1
  • Wim M. M. Schaaper
    • 2
  • Gert Wensvoort
    • 1
  1. 1.Department of VirologyInstitute for Animal Science and Health (ID-DLO)LelystadNetherland
  2. 2.Laboratory of Molecular ImmunologyInstitute for Animal Science and Health (ID-DLO)LelystadThe Netherlands

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