Induction of a Protective Immune Response to Murine Coronavirus with Non-Internal Image Anti-Idiotypic Antibodies
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Neurotropic murine coronaviruses (MHV) provide an excellent animal model to study experimental modulation of the immune response to a viral pathogen with anti-idio-typic antibodies. It is known that among the various types of anti-idiotypic antibodies (anti-Id), those designated beta (β) or internal image can molecularly mimic the antigen and induce biological activities such as anti-viral protection and neutralization. We have recently shown that polyclonal non-internal image anti-idiotypic antibodies of the β-type could induce protective anti-coronavirus immunity1.
In the present study, a polyclonal anti-Id (Ab2) was induced against a neutralizing murine monoclonal antibody (MAbl), designated 5B170.11. Mice immunized with this affinity-purified rabbit Ab2α, a non-internal image antibody, were partially protected against lethal infection by the JHM strain of MHV. However, other polyclonal and monoclonal non-internal image Ab2 induced against another neutralizing MAbl, designated 4-11G.6, were not able to protect mice against lethal infection with the A59 strain of MHV.
These results demonstrate that anti-viral protection by altering the idiotypic network with non-internal image-bearing anti-idiotype reagents can be achieved even with some anti-Id of the α-type.
KeywordsProtective Immune Response Internal Image Lethal Infection Neutralization Titer ELISA Inhibition
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