Coincidence Between Molecularly or Morphologically Classified Ectomycorrhizal Morphotypes and Fruitbodies in a Spruce Forest

  • B. Mehmann
  • S. Egli
  • G. H. Braus
  • I. Brunner
Chapter

Summary

In a study of ectomycorrhizal diversity in a spruce stand in Switzerland the macroscopically classified ectomycorrhizal morphotypes were characterised molecularly by Restriction Fragment Length Polymorphism (RFLP)-patterns of the internal transcribed spacer (ITS) region. In four different plots characterised by different levels of species diversity a total of eighteen ectomycorrhizal morphotypes were macroscopically classified. The evaluation of similarities between the different plot types revealed a more or less homogenous distribution of morphotypes which stands in contrast to the different grades of fruitbody diversity in these plots. Characterisation by molecular tools resulted in a minimum of twenty-three RFLP-types. Seven pattern-types could be referred to fungal species found as fruitbodies in the spruce stand which represents about one third of all mapped fungal species. Different types of correlation between morphotypes and fungal species were observed: (i) one morphotype represents one species, (ii) one morphotype represents several species, (iii) several morphotypes represent one species and (iv) a combination of (ii) and (iii). A low coincidence between spatial distribution and abundance of molecularly identified morphotypes and mapped fruitbodies could be found. The problems associated with classification of ectomycorrhizas and the significance of above-ground fruitbody pattern for below-ground mycorrhizal pattern is discussed.

Keywords

Internal Transcribe Spacer Fungal Species Ectomycorrhizal Fungus Reference Library Mantle Surface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Agerer, R., 1987, Colour atlas of ectomycorrhizae. Einhorn, Schwäbisch Gmünd.Google Scholar
  2. Agerer, R., 1991, Characterization of ectomycorrhiza. In: Norris, J.R., Read, D.J., and Varma, A.K. (eds.) Methods in microbiology, vol. 23. Academic Press, London, pp. 25–73.Google Scholar
  3. Brunner, I., 1991, Comparative studies on ectomycorrhizae synthesized with various in vitro techniques using Picea abies and two Hebeloma species, Trees 5:90–94.CrossRefGoogle Scholar
  4. Brunner, I., Amiet, R., and Schneider, B., 1991, Characterization of naturally grown and in vitro synthesized ectomycorrhizas of Hebeloma crustuliniforme and Picea abies, Mycol Res. 95:1407–1413.CrossRefGoogle Scholar
  5. Brunner, I., Amiet, R., Zollinger, M., and Egli, S., 1992, Ectomycorrhizal syntheses with Picea abies and three fungal species: a case study on the use of an in vitro technique to identify naturally occurring ectomycorrhizae, Mycorrhiza 2:89–96.CrossRefGoogle Scholar
  6. Christensen, M., 1981, Species diversity and dominance in fungal communities. In: Wicklow, D.T., and Caroll, G.C. (eds.) The fungal community -its organisation and role in the ecosystems. Marcel Dekker Inc., New York, pp. 201–232.Google Scholar
  7. Costa, G., and Martin, E, 1994, Directed amplification of microsatellite-region DNA in ectomycorrhizal fungi (abstract). Fourth European Symposium on Mycorrhizas, Granada, Spain, p. 14.Google Scholar
  8. Dahlberg, A., 1991, Ectomycorrhiza in coniferous forest: structure and dynamics of populations and communities. Doctoral thesis, Swedish University of Agricultural Sciences. SLU/Repro, Uppsala.Google Scholar
  9. Dahlberg, A., and Stenström, E., 1991, Dynamic changes in nursery and indigenous mycorrhiza of Pinus sylvestris seedlings planted out in forest and clearcuts, Plant Soil 136:73–86.CrossRefGoogle Scholar
  10. Danielson, R.M., and Pruden, M., 1989. The ectomycorrhizal status of urban spruce, Mycologia. 81:335–341.CrossRefGoogle Scholar
  11. Dighton, J., Poskitt, J.M., and Howard, D.M., 1986, Changes in occurrence of basidiomycete fruit bodies during forest stand development: with specific reference to mycorrhizal species, Trans. Br. Mycol. Soc. 87:163–171.CrossRefGoogle Scholar
  12. Egli, S., 1992, Der Anisklumpfuss, Cortinarius odorifer Britz.: Ökologie, Biologie und Ektomykorrhiza, Mitt. Eidg. Forschungsanst. Wald Schnee Landsch. 67:315–412.Google Scholar
  13. Egli, S., and Kälin, I., 1991, The root window -a technique for observing mycorrhizae on living trees, Agric. Ecosyst. Environ. 28:107–110.CrossRefGoogle Scholar
  14. Egli, S., Amiet, R., Zollinger, M., and Schneider, B., 1993, Characterization of Picea abies (L.) Karst. ectomycorrhizas: discrepancy between classification according to macroscopic versus microscopic features, Trees 7:123–129.CrossRefGoogle Scholar
  15. Gardes, M., and Bruns, T.D., 1993a, ITS primers with enhanced specificity for basidiomycetes -application to the identification of mycorrhizae and rusts, Mol. Ecol. 2:113–118.PubMedCrossRefGoogle Scholar
  16. Gardes, M., and Bruns, T., 1993b, The mycorrhizal guild structure of a bishop pine forest in California (abstract), In: Peterson L., Schelkle M. (eds.) Ninth North American Conference on Mycorrhizae, University of Guelph, Guelph, Ontario, Canada, p. 105.Google Scholar
  17. Gardes, M., and Bruns, T.D., 1994, ITS-RFLP matching for identification of fungi, Meth. Mol. Biol. in press.Google Scholar
  18. Gardes, M., White, T.J., Fortin, J.A., Bruns, T.D., and Taylor, J.W., 1991, Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribo-somal DNA, Can. J. Bot. 69:180–190.Google Scholar
  19. Gibson, F., and Deacon, J.W., 1988, Experimental study of establishment of ectomycorrhizas in different regions of birch root systems, Trans. Br. Mycol Soc. 91:239–251.CrossRefGoogle Scholar
  20. Haug, I., 1987, Licht-und elektronenmikroskopische Untersuchungen an Mykorrhizen von Fichtenbeständen im Schwarzwald. Dissertation, Universität Tübingen, Germany.Google Scholar
  21. Henrion, B., Chevalier, G., and Martin, F., 1994, Typing truffle species by PCR amplification of the ribosomal DNA spacers, Mycol. Res. 98:37–43.CrossRefGoogle Scholar
  22. Ingleby, K., Mason, P.A., Last, F.T., and Fleming, L.V., 1990, Identification of ectomycorrhizas, Institute of Terrestrial Ecology, Res. Publ. 5.Google Scholar
  23. Jansen, A.E., and de Vries, F.W., 1988, Dutch priority programme on acidification. Qualitative and quantitative research on the relation between ectomycorrhiza of Pseudotsuga menziesii ,vitality of the host and acid rain. Report 25–02. CEC Research contract ENV-896-NL. Comm. no. 373 of the Biological Station, Wijster, The Netherlands.Google Scholar
  24. Karen, O., Nylund, J.-E., Dahlberg, A., Högberg, N., Jonsson, L., and Grip, K., 1994, Influence of drought on mycorrhizal species composition -A comparison of identification by morphotype characters versus the PCR-technique (abstract). Fourth European Symposium on Mycorrhizas, Granada, Spain, p. 19.Google Scholar
  25. Kottke, I., 1986, Charakterisierung und Identifizierung von Mykorrhizen. In: Einsele, G. (ed.) Das landschaftsökologische Forschungsprojekt Naturpark Schönbuch (Forschungsbericht). Deutsche Forschungsgemeinschaft, Weinheim, pp. 463–485.Google Scholar
  26. Lanfranco, L., Arlorio, M., Matteucci, A., and Bonfante, P., 1994, Truffles: Life cycle and molecular characterization (abstract), International Symposium on Biotechnology of Ectomycorrhizae: Molecular Approaches, Urbino, Italy, pp. 34–35.Google Scholar
  27. Mehmann, B., Brunner, I., and Braus, G.H., 1994, Nucleotide Sequence Variation of Chitin Synthase Genes among Ectomycorrhizal Fungi and its Potential Use in Taxonomy, Appl. Environ. Microbiol 60:3105– 3111.PubMedGoogle Scholar
  28. Menge, J.A., and Grand, L.F., 1978, Effect of fertilization on production of epigeous basidiocarps by mycorrhizal fungi in loblolly pine plantations. Can. J. Bot. 56:2357–2362.CrossRefGoogle Scholar
  29. Moser, M., 1983, Die Röhrlinge und Blätterpilze. Kleine Kryptogamenflora IIb/2. Gustav Fischer Verlag, Stuttgart, Germany.Google Scholar
  30. Palenzona, M., and Fontana, A., 1970, Influenza di tipi di suolo su tre forme micorrhiziche del pino strobo, Allionia 16:101–113.Google Scholar
  31. Potenza, L., Amicucci, A., Rossi, I., Palma, F., De Bellis, R., Cardoni, P., and Stocchi, V, 1994a, Identification of Tuber magnatum Pico DNA markers by RAPD analysis, Biotechnology Techniques 8:93–98.CrossRefGoogle Scholar
  32. Potenza, L., Amicucci, A., Rossi, L, Palma, F., De Bellis, R., Cardoni, P., and Stocchi, V., 1994b, Identification of genetic markers for the characterization of Tuber species (abstract), International Symposium on Biotechnology of Ectomycorrhizae: Molecular Approaches, Urbino, Italy, pp. 55–56.Google Scholar
  33. Pritsch, K., and Buscot, F., 1994, Biodiversity of ectomycorrhizas -from morphotypes to species, (abstract). Fourth European Symposium on Mycorrhizas, Granada, Spain, p. 10.Google Scholar
  34. Read, D.F., 1984, The structure and function of the vegetative mycelium of mycorrhizal roots. In: Jennings, D.H., Rayner, A.D.M., (eds.) Methods in microbiology, vol. 23. Academic Press, London, UK, pp. 253–281.Google Scholar
  35. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B., and Ehrlich, H.A., 1988, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase, Science 239:487–491.PubMedCrossRefGoogle Scholar
  36. Taylor, A.F.S., and Alexander, I.J., 1990, Demography and population dynamics of ectomycorrhizas of sitka spruce fertilized with N, Agric. Ecosyst. Environ. 28:493–496.CrossRefGoogle Scholar
  37. Thoen, D., 1977, Identification of ectomycorrhizal fungi by thin layer chromatography, Bull. Br. Mycol. Soc. 11:39–43.CrossRefGoogle Scholar
  38. Voiry, H., 1981, Classification morphologique des ectomycorhizes du chêne et du hêtre dans le nord-est de la France, Eur. J. For. Pathol. 11:284–299.CrossRefGoogle Scholar
  39. Wyss, P., and Bonfante, P., 1993, Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers, Mycol. Res. 97:1351–1357.CrossRefGoogle Scholar
  40. Zak, B., 1973, Classification of ectomycorrhizae. In: Marks G.C., Kozlowski T.T. (eds.) Ectomycorrhizae, Academic Press, New York, pp. 43–78.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • B. Mehmann
    • 1
  • S. Egli
    • 1
  • G. H. Braus
    • 2
  • I. Brunner
    • 1
  1. 1.Swiss Federal Institute for Forest, Snow and Landscape Research (WSL)BirmensdorfSwitzerland
  2. 2.Institute of Microbiology, Biochemistry and GeneticsFriedrich-Alexander-UniversityErlangenGermany

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