Advertisement

Glucose Diffusion Coefficients Determined from Concentration Profiles in Emt6 Tumor Spheroids Incubated in Radioactively Labeled L-Glucose

  • K. Groebe
  • S. Erz
  • W. Mueller-Klieser
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 361)

Abstract

In order to theoretically assess tissue energetic status, conditions for substance exchange need to be known. One group of parameters important in this context are diffusion coefficients of nutrients and metabolic waste products which may be assessed by incubating spheroids in a medium containing tracer amounts of the radioactively labeled substance in question, for a defined period of time. In previous studies, the overall amount of 14C-labeled substance taken up by the spheroids was measured by scintillation counters (e.g.[1]), or the concentration of 3H-labeled substance in the spheroid center was determined by autoradiography and grain counting (e.g. [4]). From a number of such measurements, diffusion coefficients may then be obtained using mathematical models. The information content of an autoradiogram can be exploited more efficiently by recording not only central grain density but rather grain density distributions from each of which a diffusion coefficient may be calculated. This can be done by grain counting on a radial track through an autoradiogram of a central spheroid section for 3 H labels or by measuring optical density (densitometry) in autoradiograms of central spheroid sections for 14 C labels.

Keywords

Tumor Spheroid Short Incubation Time Multicellular Spheroid Multicellular Tumor Spheroid Metabolic Waste Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    CASCIARI, J. J., S. V. SOTIRCHOS, and R. M. SUTHERLAND, Glucose diffusivity in multicellular tumor spheroids. Cancer Res. 48:3905–3909 (1988)PubMedGoogle Scholar
  2. [2]
    CASCIARI, J. J., S. V. SOTIRCHOS, and R. M. SUTHERLAND, Variations in tumor cell growth rates and metabolism with oxygen concentration, glucose concentration, and extracellular pH. J. Cell. Physiol. 151:386–394 (1992)PubMedCrossRefGoogle Scholar
  3. [3]
    CASCIARI, J. J., S. V.SOTIRCHOS, and R. M. SUTHERLAND, Mathematical modelling of microenvironment and growth in EMT6/Ro multicellular tumour spheroids. Cell Prolif. 25:1–22 (1992)PubMedCrossRefGoogle Scholar
  4. [4]
    DOERSCHEL, D., U. KARBACH, GROEBEK., and W. MUELLER-KLIESER, Assessment of glucose penetration into tumor microregions by high resolution autoradiography 37th Meeting of the European Tissue Culture Society, Graz, Austria, 1989. Cylolechnology 2 (Suppl.):36 (1989)Google Scholar
  5. [5]
    FREYER, J. P. and R. M. SUTHERLAND, Determination of diffusion constants for metabolites in multicell tumor spheroids, Adv.Erp.Med.Biol. 159:463–475 (1983)CrossRefGoogle Scholar
  6. [6]
    GROEBEK. and W.MUELLER-KLIESER, Distributions of oxygen, nutrient, and metabolic waste concentrations in multicellular spheroids and their dependence on spheroid parameters, Eur.Biophys.J. 19:169–181 (1991)PubMedCrossRefGoogle Scholar
  7. [7]
    LI, C. K. N., The glucose distribution in 9L rat brain multicell tumor spheroids and its effect on cell necrosis, Cancer (Phila.) 50:2066–2073 (1982)CrossRefGoogle Scholar
  8. [8]
    MUELLER-KLIESER, W., Multicellular spheroids. A review on cellular aggregates in cancer research, J. Cancer Res. Clin. Oncol. 113:101–122 (1987)PubMedCrossRefGoogle Scholar
  9. [9]
    SUTHERLAND, R. M., Cell and environment interactions in tumor microregions: the multicell spheroid model, Science 240:177–184 (1988)PubMedCrossRefGoogle Scholar
  10. [10]
    SWABB, E. A., J. WEI, and P. M.GULLING, Diffusion and convection in normal and neoplastic tissues, Cancer Res. 34:2814–2822 (1974)PubMedGoogle Scholar
  11. [11]
    VAUPEL, P., Oxygenation of human tumors, Strahlenther. Onkol. 166:377–386 (1990)PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • K. Groebe
    • 1
  • S. Erz
    • 1
  • W. Mueller-Klieser
    • 1
  1. 1.Institut für Physiologie und PathophysiologieJohannes Gutenberg- Universität MainzMainzGermany

Personalised recommendations