Cloning and Identification of MARC-145 Cell Proteins Binding to 3’ UTR and Partial Nucleoprotein Gene of Porcine Reproductive and Respiratory Syndrome Virus
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The PRRSV has a (+) sense RNA genome 15 kb in size, with 5′ and 3′ untranslated regions (UTR) of 156-220 and 59-117 nucleotides flanking the viral genome (Snijder and Meulenberg, 1998). Virus grows in MARC, CL2621 and MA-104 cell lines. BHK-21 cells when transfected with PRRSV RNA, produced new infectious virus (Meulenberg et al., 1998). PRRSV enters cells by standard endocytosis route and low pH in these vesicles is required for the viral entry (Kreutz and Ackermann, 1996).
KeywordsRespiratory Syndrome Virus Host Cell Protein Equine Arteritis Virus Infectious cDNA Clone Upstream Regulatory Sequence
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