Cloning and Identification of MARC-145 Cell Proteins Binding to 3’ UTR and Partial Nucleoprotein Gene of Porcine Reproductive and Respiratory Syndrome Virus

  • Kumar Shanmukhappa
  • Sanjay Kapil
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)


The PRRSV has a (+) sense RNA genome 15 kb in size, with 5′ and 3′ untranslated regions (UTR) of 156-220 and 59-117 nucleotides flanking the viral genome (Snijder and Meulenberg, 1998). Virus grows in MARC, CL2621 and MA-104 cell lines. BHK-21 cells when transfected with PRRSV RNA, produced new infectious virus (Meulenberg et al., 1998). PRRSV enters cells by standard endocytosis route and low pH in these vesicles is required for the viral entry (Kreutz and Ackermann, 1996).


Respiratory Syndrome Virus Host Cell Protein Equine Arteritis Virus Infectious cDNA Clone Upstream Regulatory Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Ashman, L. K., G. W. Aylett, P. A. Mehrabani, L. J. Bendall, S. Niutta, A. C. Cambaren, S. R. Cole, and M. C. Berndt. 1991. The murine monoclonal antibody, 14A2.H1, identifies a novel platelet surface antigen. Br J Haematol. 79:263–270.PubMedCrossRefGoogle Scholar
  2. Fitter, S., T. J. Tetaz, M. C. Berndt, and L. K. Ashman. 1995. Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3. Blood. 86:1348–1355.PubMedGoogle Scholar
  3. Hasegawa, H., Y. Utsunomiya, K. Kishimoto, K. Yanagisawa, and S. Fujita. 1996. SFA-1, a novel cellular gene induced by human T-cell leukemia virus type 1, is a member of the transmembrane 4 superfamily [published erratum appears in J Virol 1997 Feb;71 (2): 1737]. J Virol. 70:3258–3263.PubMedGoogle Scholar
  4. Kreutz, L. C., and M. R. Ackermann. 1996. Porcine reproductive and respiratory syndrome virus enters cells through a low pH-dependent endocytic pathway. Virus Res. 42:137–147.PubMedCrossRefGoogle Scholar
  5. Lin, Y. J., X. Zhang, R. C. Wu, and M. M. Lai. 1996. The 3′ untranslated region of Coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA. J Virol. 70:7236–7240.PubMedGoogle Scholar
  6. Meulenberg, J. J., J. N. Bos-de Ruijter, G. Wensvoort, and R. J. Moormann. 1998. An infectious cDNA clone of porcine reproductive and respiratory syndrome virus. Adv Exp Med Biol. 440:199–206.PubMedCrossRefGoogle Scholar
  7. Pedersen, K. W., Y. van der Meer, N. Roos, and E. J. Snijder. 1999. Open reading frame la-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex. J Virol. 73:2016–2126.PubMedGoogle Scholar
  8. Sagesser, R., E. Martinez, M. Tsagris, and M. Tabler. 1997. Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library. Nucleic Acids Res. 25:3816–3822.PubMedCrossRefGoogle Scholar
  9. Sincock, P. M., S. Fitter, R. G. Parton, M. C. Berndt, J. R. Gamble, and L. K. Ashman. 1999. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. J Cell Sci. 112:833–844.PubMedGoogle Scholar
  10. Sincock, P. M., G. Mayrhofer, and L. K. Ashman. 1997. Localization of the transmembrane 4 superfamily (TM4SF) member PETA-3 (CD151) in normal human tissues: comparison with CD9, CD63, and alpha5betal integrin. J Histochem Cytochem. 45:515–525.PubMedCrossRefGoogle Scholar
  11. Snijder, E. J., and J. J. Meulenberg. 1998. The molecular biology of arteriviruses. J Gen Virol. 79:961–979.PubMedGoogle Scholar
  12. Zhang, X., C. L. Liao, and M. M. Lai. 1994. Coronavirus leader RNA regulates initiates subgenomic mRNA transcription both in trans and in cis. J Virol. 68:4738–4746.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Kumar Shanmukhappa
    • 1
  • Sanjay Kapil
    • 1
  1. 1.Department of Diagnostic Medicine -Pathobiology, College of Veterinary MedicineKansas State UniversityManhattanUSA

Personalised recommendations