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Use of Defective RNAs Containing Reporter Genes to Investigate Targeted Recombination for Avian Infectious Bronchitis Virus

  • Benjamin Neuman
  • Dave Cavanagh
  • Paul Britton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

At present there is no infectious cDNA of IBV and the robustness of Coronavirus cDNA reverse genetic systems remains undetermined. We have been investigating an alternate strategy for reverse genetic manipulation of IBV using defective RNAs (D-RNAs) to exchange genetic material with the IBV genome, based on efficient recombination systems for other coronaviruses. It has been previously shown that two strains of IBV can recombine in ovo (Kottier et al., 1995) though recombination has not yet been demonstrated in cell culture. IBV D-RNA CD-91 (Pénzes et al., 1994) consisting of regions from the 5’-end, open reading frame 1b and the 3’-end including most of the N gene was isolated and cloned after high multiplicity passage of IBV Beaudette. Internal sequences were deleted from CD-91 to generate CD-61 (Pénzes et al., 1996). Both D-RNAs contain all the necessary signals for replication and packaging (rescue) by helper IBV.

Keywords

Gene Cassette Plaque Assay Infectious Bronchitis Virus EGFP Gene Reverse Genetic System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Benjamin Neuman
    • 1
  • Dave Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Division of Molecular BiologyInstitute for Animal Health, Compton LaboratoryCompton, Newbury, BerkshireUK

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