A Simple Strategy to Assemble Infectious RNA and DNA Clones

  • Kristopher M. Curtis
  • Boyd Yount
  • Ralph S. Baric
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)


The availability of infectious full-length cDNA clones is important for the molecular genetic analysis of the structure and function of RNA virus genomes (Ahlquist et al, 1984; Boursnell et al., 1987). Infectious cDNA clones for a number of positive-stranded RNA viruses have been developed, advancing our understanding of the molecular mechanisms of viral replication and pathogenesis, and has resulted in novel approaches for heterologous gene expression and vaccine development. Clearly, a full-length infectious construct of TGEV would enhance our understanding of all aspects of TGEV biology by providing a means for reverse genetic analysis.


Infectious Bronchitis Virus Infectious Clone Baby Hamster Kidney Cell Infectious cDNA Clone Transmissible Gastroenteritis Virus 
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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Kristopher M. Curtis
    • 1
  • Boyd Yount
    • 2
  • Ralph S. Baric
    • 1
    • 2
  1. 1.Department of Microbiology and ImmunologySchool of MedicineUSA
  2. 2.Department of Epidemiology, Program of Infectious DiseasesSchool of Public Health, University of North Carolina at Chapel HillChapel HillUSA

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