Regulation of Mouse Hepatitis Virus RNA synthesis by Heterogeneous Nuclear Ribonucleoprotein Al

  • Stephanie T. Shi
  • Peiyong Huang
  • Hsin-Pai Li
  • Michael M. C. Lai
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)


Mouse hepatitis virus (MHV) RNA synthesis, including replication of viral genome and transcription of subgenomic mRNAs, has been shown to be regulated by several viral RNA elements, including 5’-untranslated region (5’-UTR), cis- and trans-acting leader RNA, intergenic (IG) sequence, and 3’-UTR (Lai and Cavanagh 1997). Biochemical evidence suggests that these regulatory sequences likely interact with each other either directly or indirectly, probably through protein-RNA and protein-protein interactions involving both viral and cellular proteins (Zhang and Lai 1995). Several cellular proteins have been found to bind these regulatory sequences by UV-crosslinking experiments (Furuya and Lai 1993, Huang and Lai 1999); three of these proteins have been identified to be heterogeneous nuclear ribonucleoprotein Al (hnRNP A1) (Li et al 1997), polypyrimidine-tractbinding protein (PTB) (Huang and Lai 1999, Li et al 1999), and poly(A)-binding protein (PABP) (Spagnolo and Hogue 2000).


Heterogeneous Nuclear Ribonucleoprotein Mouse Hepatitis Virus Howard Hughes Medical Institute hnRNP Protein Subgenomic mRNA 
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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Stephanie T. Shi
    • 1
  • Peiyong Huang
    • 1
  • Hsin-Pai Li
    • 1
  • Michael M. C. Lai
    • 1
    • 2
  1. 1.Department of Molecular Microbiology and ImmunologyUniversity of Southern California School of MedicineLos AngelesUSA
  2. 2.Howard Hughes Medical InstituteUniversity of Southern California School of MedicineLos AngelesUSA

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