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A Virus-Neutralising Monoclonal Antibody Expressed in the Milk of Transgenic Mice

  • A. F. Kolb
  • J. Webster
  • C. B. A. Whitelaw
  • S. G. Siddell
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Coronaviruses are frequently associated with respiratory and gastrointestinal disorders in both animals and man. In adult animals, Coronavirus infections are generally mild, however, in neonates they often cause severe and sometimes lethal diseases (Enjuanes and van der Zeijst, 1995). Maternal antibodies supplied via the placenta and milk efficiently protect new-born animals against the fatal consequences of acute coronaviral infections during this critical phase (Hornberger, 1992).

Keywords

Transgenic Mouse Recombinant Antibody Trans Gene Heavy Chain Variable Region Antibody Light Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Castilla, J., Pintado, B., Sola, I., Sanchez, M. J. and Enjuanes, L., 1998, Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk. Nat. Biotechnol. 16, 349–354PubMedCrossRefGoogle Scholar
  2. Clark, A. J., Cowper, A., Wallace, R., Wright, G. and Simons, J. P., 1992, Rescuing transgene expression by co-integration. Biotechnology 10, 1450–1454PubMedCrossRefGoogle Scholar
  3. Enjuanes L. and van der Zeijst B. A. M., 1995, Molecular basis of transmissible gastroenteritis virus epidemiology. In The Coronaviridae (Siddell, S. G. ed.), Plenum Press, New York, pp. 337–376Google Scholar
  4. Hornberger, F. R., 1992, Maternally-derived passive immunity to enterotropic mouse hepatitis virus. Arch. Virol. 122, 133–141CrossRefGoogle Scholar
  5. Hornberger, F. R., Barthold, S. W. and Smith, A. L., 1992, Duration and strain-specificity of immunity to enterotropic mouse hepatitis virus. Lab. Anim. Sci. 42, 347–351Google Scholar
  6. Kolb, A. F. and Siddell, S. G., 1997, Expression of a recombinant monoclonal antibody from a bicistronic mRNA. Hybridoma 16, 421–426PubMedCrossRefGoogle Scholar
  7. Saif, L. and Wheeler, M. B., 1998, WAPing gastroenteristis with transgenic antibodies. Nat. Biotechnol. 16,334–335PubMedCrossRefGoogle Scholar
  8. Sola, I., Castilla, J., Pintado, B., Sanchez, M. J., Whitelaw, C. B., Clark, A. J. and Enjuanes, L., 1998, Transgenic mice secreting Coronavirus neutralizing antibodies into the milk. J. Virol. 72, 3762–3772PubMedGoogle Scholar
  9. Wege, H., Dömes, R. and Wege, H., 1984, Hybridoma antibodies to the murine Coronavirus JHM: characterization of epitopes on the peplomer protein E2. J. of Gen. Virol. 65, 1913–1941Google Scholar
  10. Whitelaw, C. B. A., Harris, S., McClenaghan, M., Simons, J. P. and Clark, A. J., 1992. Position-independent expression of the ovine beta-lactoglobulin gene in transgenic mice. Biochem. J. 286,31–39PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • A. F. Kolb
    • 1
    • 2
  • J. Webster
    • 3
  • C. B. A. Whitelaw
    • 3
  • S. G. Siddell
    • 2
  1. 1.Hannah Research InstituteCell Physiology GroupAyrUK
  2. 2.Institute of VirologyUniversity of WürzburgWürzburgGermany
  3. 3.Division of Molecular BiologyRoslin InstituteRoslinUK

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