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Enhancement of Defective RNA Expression Vectors as Potential Vaccine Delivery Systems for Avian Infectious Bronchitis Virus

  • Brian Dove
  • Kathy Shaw
  • Julian Hiscox
  • David Cavanagh
  • Paul Britton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

IBV defective RNA (D-RNA) CD-61, a deletion mutant of the naturally occurring D-RNA CD-91, is capable of being utilised as an RNA expression vector for heterologous genes (Pénzes et al., 1996; Stirrups et al., 2000a; Stirrups et al., 2000b). The reporter genes chloramphenicol acetyltransferase (CAT) and luciferase, under the control of IBV gene 5 transcription associated sequence (TAS), are capable of expression from within CD-61 during D-RNA rescue. However the modification of CD-61 by the insertion of foreign gene sequences has an adverse effect on D-RNA rescue efficiency resulting in an eventual loss of foreign gene expression (Stirrups et al., 2000b). We attempted to enhance heterologous gene expression in CD-61 by utilising the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) as an alternative to gene 5 TAS to control CAT gene expression.

Keywords

Internal Ribosome Entry Site Cell Free System Infectious Bronchitis Virus Rabbit Reticulocyte Lysate Heterologous Gene Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Pénzes, Z., Wroe, C., Brown, T. D., Britton, P. & Cavanagh, D. 1996. Replication and packaging of Coronavirus infectious bronchitis virus defective RNAs lacking a long open reading frame. J. Virol. 70, 8660–8668.PubMedGoogle Scholar
  2. Stirrups, K., Shaw, K., Evans, S., Dalton, K., Cavanagh, D. & Britton, P. 2000a. Leader switching occurs during the rescue of defective RNAs by heterologous strains of the Coronavirus infectious bronchitis virus (IBV). J. Gen, Virol. 81, 791–801.Google Scholar
  3. Stirrups, K., Shaw, K., Evans, S., Dalton, K., Casais, R., Cavanagh, D. & Britton, P. 2000b. Expression of reporter genes from the defective RNA CD-61 of the Coronavirus infectious bronchitis virus. J. Gen. Virol. 81, 1687–1698.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Brian Dove
    • 1
  • Kathy Shaw
    • 1
  • Julian Hiscox
    • 1
  • David Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Division of Molecular BiologyInstitute for Animal Health, Compton LaboratoryCompton, Newbury, BerkshireUK

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