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Regulation of Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Matrix Metalloproteinase (TIMP) Genes During JHMV Infection of the Central Nervous System

  • Jiehao Zhou
  • Stephen A. Stohlman
  • Norman W. Marten
  • David R. Hinton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

The central nervous system (CNS) is refractive to many aspects of the immune system primarily due to its limited ability to repair damage induced by the cytopathic mechanisms deployed by most immune cells. A primary obstacle to CNS inflammation is the blood-brain-barrier (BBB), which limits entry of immune cells into the CNS. To pass the BBB, inflammatory cells release matrix metalloproteinases (MMPs); a growing family of proteases with overlapping substrate specificities for components of the extracellular matrix (reveiwed by Kieseier et al. 1999). MMPs break down the extracellular matrix surrounding the endothelial layer of BBB thereby permitting peripheral immune cells to traverse the BBB and migrate through the parenchyma of the CNS in response to inflammatory signals. To limit potential damage resulting from infiltration of inflammatory cells, MMP activity is tightly regulated at both the level of gene expression and proenzyme activation as well as by expression of a second gene family, the tissue inhibitors of MMPs (TIMPs). TIMPs act as competitive inhibitors for the active sites of MMPs and thus limit inflammatory infiltrates.

Keywords

RNAse Protection Assay Mononuclear Cell Infiltration Immune Cell Infiltration naIve Mouse Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Jiehao Zhou
    • 1
  • Stephen A. Stohlman
    • 2
    • 3
  • Norman W. Marten
    • 1
  • David R. Hinton
    • 1
  1. 1.Department of PathologyUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.Department of NeurologyUniversity of Southern CaliforniaLos AngelesUSA
  3. 3.Molecular Microbiology and Immunology Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA

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