Exploiting DNA Immunization to Generate Polyclonal Antisera to Coronavirus Replicase Proteins
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Over the last 10 years, a large number of studies have shown that immunization with plasmid DNA encoding an antigen can elicit specific immune responses in animals (reviewed in Donnelly et al 1997; Robinson and Torres 1997). The type of immune response generated depends on several factors, such as the route of inoculation, whether the DNA-encoded antigen remains intracellular or is secreted, and the stability and antigenicity of the protein products. DNA immunization is an attractive alternative to traditional purified protein immunizations because plasmid DNA is relatively easy and inexpensive to generate. However, additional studies are required to determine the optimal route of delivery of plasmid DNA and if additional factors may help “direct” the encoded antigen to elicit a specific type of immune response.
KeywordsPolyclonal Antiserum Mouse Hepatitis Virus Specific Antibody Response Murine Coronavirus Strong Humoral Immune Response
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