Addition of a Single Glycosylation Site to hAPN Blocks Human Coronavirus-229E Receptor Activity

  • David E. Wentworth
  • Kathryn V. Holmes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)


The cellular receptor for HCoV-229E is human aminopeptidase N (hAPN). Murine fibroblasts that are nonpermissive for HCoV-229E become susceptible after transfection with an hAPN expression plasmid (Yeager et al., 1992). In addition, antibodies to hAPN block infection of human neural cells by HCoV-229E (Lachance et al., 1998). hAPN, also called CD13, is a 150 kDa glycoprotein that is a membrane peptidase (Look et al., 1989). APN is expressed by many cell types including epithelial cells of the kidney, intestine, respiratory tracts and at synaptic junctions in the CNS (Kenny and Maroux, 1982; Look et al., 1989; Riemann et al., 1999; Noren et al., 1997).


Glycosylation Site Feline Infectious Peritonitis Feline Infectious Peritonitis Virus Human Neural Cell CMT93 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Delmas, B., Gelfi, J., Kut, E., Sjostrom, H., Noren, O., and Laude, H. (1994). Determinants essential for the transmissible gastroenteritis virus-receptor interaction reside within a domain of aminopeptidase-N that is distinct from the enzymatic site. Journal of Virology 68, 5216–5224.PubMedGoogle Scholar
  2. Delmas, B., Gelfi, J., L’Haridon, R., Vogel, L. K., Sjostrom, H., Noren, O., Laude, H. (1992). Aminopeptidase N is a major receptor for the entero-pathogenic Coronavirus TGEV. Nature 357, 417–420.PubMedCrossRefGoogle Scholar
  3. Delmas, B., Gelfi, J., Sjostrom, H., Noren, O., and Laude, H. (1993). Further characterization of aminopeptidase-N as a receptor for coronaviruses. Advances in Experimental Medicine & Biology 342, 293–298.CrossRefGoogle Scholar
  4. Hegyi, A. and Kolb, A. F. (1998). Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N. Journal of General Virology 79, 1387–1391.PubMedGoogle Scholar
  5. Kenny, A. J. and Maroux, S. (1982). Topology of microvillar membrance hydrolases of kidney and intestine. [Review] [153 refs]. Physiological Reviews 62, 91–128.PubMedGoogle Scholar
  6. Kolb, A. F., Hegyi, A., and Siddell, S. G. (1997). Identification of residues critical for the human Coronavirus 229E receptor function of human aminopeptidase N. Journal of General Virology 78, 2795–2802.PubMedGoogle Scholar
  7. Lachance, C., Arbour, N., Cashman, N. R., and Talbot, P. J. (1998). Involvement of aminopeptidase N (CD13) in infection of human neural cells by human Coronavirus 229E. Journal of Virology 72, 6511–6519.PubMedGoogle Scholar
  8. Look, A. T., Ashmun, R. A., Shapiro, L. H., and Peiper, S. C. (1989). Human myeloid plasma membrane glycoprotein CD13 (gpl50) is identical to aminopeptidase N. Journal of Clinical Investigation 83, 1299–1307.PubMedCrossRefGoogle Scholar
  9. Noren, O., Sjostrom, H., and Olsen, J. (1997). Cell-Surface Peptidases in Health and Disease (Kenney, A. J. and Boustead, C. M., Eds.) BIOS Scientific Publishers, Oxford. 175–191.Google Scholar
  10. Riemann, D., Kehlen, A., and Langner, J. (1999). CD 13 — not just a marker in leukemia typing [Review]. Immunology Today 20, 83–88.PubMedCrossRefGoogle Scholar
  11. Tresnan, D. B., Levis, R., and Holmes, K. V. (1996). Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. Journal of Virology 70, 8669–8674.PubMedGoogle Scholar
  12. Yeager, C. L., Ashmun, R. A., Williams, R. K., Cardellichio, C. B., Shapiro, L. H., Look, AT, and Holmes, K. V. (1992). Human aminopeptidase N is a receptor for human Coronavirus 229E. Nature 357, 420–422.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • David E. Wentworth
    • 1
  • Kathryn V. Holmes
    • 1
  1. 1.Department of MicrobiologyUniversity of Colorado Health Sciences CenterDenverUSA

Personalised recommendations