Addition of a Single Glycosylation Site to hAPN Blocks Human Coronavirus-229E Receptor Activity
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The cellular receptor for HCoV-229E is human aminopeptidase N (hAPN). Murine fibroblasts that are nonpermissive for HCoV-229E become susceptible after transfection with an hAPN expression plasmid (Yeager et al., 1992). In addition, antibodies to hAPN block infection of human neural cells by HCoV-229E (Lachance et al., 1998). hAPN, also called CD13, is a 150 kDa glycoprotein that is a membrane peptidase (Look et al., 1989). APN is expressed by many cell types including epithelial cells of the kidney, intestine, respiratory tracts and at synaptic junctions in the CNS (Kenny and Maroux, 1982; Look et al., 1989; Riemann et al., 1999; Noren et al., 1997).
KeywordsGlycosylation Site Feline Infectious Peritonitis Feline Infectious Peritonitis Virus Human Neural Cell CMT93 Cell
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