Neurovirulence for Mice of Soluble Receptor-Resistant Mutants of Murine Coronavirus JHMV
Part of the
Advances in Experimental Medicine and Biology
book series (AEMB, volume 494)
Soluble receptor-resistant (srr) mutants isolated from a highly neurotropic mouse hepatitis virus (MHV), JHMV cl-2 strain, have a single mutation in either SI or S2 subunit of spike (S) protein (Saeki et al., 1997). These mutants were shown to grow and induce syncytia in cultured cells as efficiently as wild type (wt) virus (Saeki et al., 1997). Since it has been reported that the S protein is a key determinant in the viral virulence for animals (Dalziel et al., 1986; Fleming et al., 1986; Philips et al., 1999), we have attempted to see whether the mutations observed in srr mutants affect the neurovirulence of JHMV.
KeywordsMouse Hepatitis Virus Spike Protein Central Nervous System Symptom Murine Hepatitis Virus Single Amino Acid Mutation
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