Spread of Hemagglutinating Encephalomyelitis Virus (HEV) in the CNS of Rats Inoculated by Intranasal Route

  • Norio Hirano
  • Koujiro Tohyama
  • Hideharu Taira
  • Tsutomu Hashikawa
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)


HEV is known to cause vomiting and wasting disease or encephalomyelitis in piglets (Andries et al., 1980,1981; Mengeling et al., 1972; Roe et al., 1958). In experimental oronasal infection of piglets, the virus spread to the CNS predominantly via the nerve pathways (Andries et al., 1980, 1981). In our previous study, 4-week-old rats died of encephalitis after inoculation by different routes including intranasal route (Hirano et al., 1993). In the infected animals, the virus reaches the CNS through the nerve pathways from peripheral nerve. Our previous studies have demonstrated that HEV propagated through nervous route and its infection was restricted to neurons after inoculation into sciatic nerve or footpad of rats (Hirano et al., 1995, 1998). Our findings suggest that HEV is useful as a trans-synaptic tracer for analyzing neuronal connections in the CNS.


Dentate Gyrus Superior Colliculus Cerebellar Cortex Inferior Colliculus Positive Neuron 
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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Norio Hirano
    • 1
  • Koujiro Tohyama
    • 3
    • 4
  • Hideharu Taira
    • 2
  • Tsutomu Hashikawa
    • 4
  1. 1.Department of Veterinary MicrobiologyIwate UniversityMoriokaJapan
  2. 2.Department of Bioscience and TechnologyIwate UniversityMoriokaJapan
  3. 3.The Center for Electron Microscopy and Bio-imaging Research and Department of NeuroanatomyIwate Medical UniversityMoriokaJapan
  4. 4.Laboratory of Neural ArchitectureBrain Science Institute, RIKENWakoJapan

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