Caspase Inhibitors Block MHV-3 Induced Apoptosis and Enhance Viral Replication and Pathogenicity
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Infection with mouse hepatitis virus strain 3 (MHV-3) results in lethal hepatitis in BALB/c mice, compared to the minimal disease observed in A/J mice (Levy, Leibowitz, and Edgington, 1981). The response of the macrophage to MHV infection is a key determinant of the outcome of MHV-induced hepatitis (Levy, Leibowitz, and Edgington, 1981; Shif and Bang, 1969). We have previously shown that infection of A/J macrophages with MHV-3 triggers apoptosis in most of the infected cells (Belyavskyi et al., 1998). In contrast, infection of BALB/c macrophages results in only a small percentage of the cells undergoing apoptosis. In this report we have utilized caspase inhibitors to investigate the hypothesis that the rapid induction of apoptosis in macrophages derived from A/J mice limits MHV replication and hepatic injury during MHV infection.
KeywordsCaspase Inhibitor Infected Macrophage Viral Yield Mouse Hepatitis Virus Murine Hepatitis Virus
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