Waveguide Fluorosensor for the Detection of Ligand-Receptor Interactions

The 5-HT3 Serotonin Receptor
  • Evelyne L. Schmid
  • Ruud Hovius
  • Michael Pawlak
  • Markus Ehrat
  • Horst Vogel

Abstract

A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent ligand were evaluated. The approach is particularly suited for the study of rapid ligand-receptor interactions where optimized cell volume, flow and injection conditions are required.

Keywords

serotonin-gated ion channel membrane receptor biosensor fluorescence detection functional immobilization ligand binding 

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Evelyne L. Schmid
    • 1
  • Ruud Hovius
    • 1
  • Michael Pawlak
    • 2
  • Markus Ehrat
    • 2
  • Horst Vogel
    • 1
  1. 1.Laboratoire de Chimie Physique des Polymères et MembranesEcole Polytechnique Fédérale de LausanneLausanneSwitzerland
  2. 2.Zeptosens AGWitterswilSwitzerland

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