Waveguide Fluorosensor for the Detection of Ligand-Receptor Interactions
The 5-HT3 Serotonin Receptor
Chapter
Abstract
A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent ligand were evaluated. The approach is particularly suited for the study of rapid ligand-receptor interactions where optimized cell volume, flow and injection conditions are required.
Keywords
serotonin-gated ion channel membrane receptor biosensor fluorescence detection functional immobilization ligand bindingPreview
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