Biology and Ecology of Mycotoxigenic Aspergillus Species as Related to Economic and Health Concerns

  • David M. Wilson
  • Wellington Mubatanhema
  • Zeljko Jurjevic
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 504)


The fungal genusAspergilluswas established in 1729, and includes species that are adapted to a wide range of environmental conditions. Many aspergilli produce mycotoxins in foods that may be toxic, mutagenic or carcinogenic in animals. Most of the Aspergillus species are soil fungi or saprophytes but some are capable of causing decay in storage, disease in plants or invasive disease in humans and animals. Major agricultural commodities affected before or after harvest by fungal growth and mycotoxins include corn, peanuts, cottonseed, rice, tree nuts, cereal grains, and fruits. Animal products (meat, milk and eggs) can become contaminated because of diet.Aspergillus flavus,A. parasiticus,A. ochraceus,A. niger,A. fumigatusand other aspergilli produce mycotoxins of concern. These include the aflatoxins and ochratoxins, as well as cyclopiazonic acid, patulin, sterigmatocystin, gliotoxin, citrinin and other potentially toxic metabolites.


Aspergillus Species Aflatoxin Production Aflatoxin Contamination Cyclopiazonic Acid Mycotoxin Contamination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Abarca, M.L., Bragulat, M.R., Castella, G., and Cabanes, F.J., 1994, Ochratoxin production by strains ofAspergillus niger var. niger,Appl. Env. Microbiol., 60:2650.Google Scholar
  2. Abramson, D., 1991, Development of molds, mycotoxins and odors in moist cereals, during storage in:Cereal Grain Mycotoxins,Fungi and Quality in Drying and Storage, Chelkowski, J., ed. Elsevier, Amsterdam.Google Scholar
  3. Abramson, D., Sinha, R.N. and Mills, J.T., 1983, Mycotoxin and odor formation in barley stored at 16 and 20% moisture in Manitoba.Cereal Chem.60:350.Google Scholar
  4. Beti, J.A., Phillips, T.W. and Smalley, E.B., 1995, Effects of maize weevils (Coleoptora:Curculionidae) on production of aflatoxin B1byAspergillus flavusin stored corn,Stored-Product Entomol., 88:1776.Google Scholar
  5. Bucheli, P., Kanchanomai, C., Meyer, L., and Pittet, A., 2000, Development of ochratoxin A during robusta(Coffee canephora)coffee cherry drying,J. Agric. Food Chem., 48:1358.CrossRefGoogle Scholar
  6. Calvert, O.H., Lillehoj, E.B., Kwolek, W.F., and Zuber, M.S., 1978, Aflatoxin B1and G1production in developingZea mayskernels from mixed inocula ofAspergillus flavusandA. parasiticus. Phytopathology68:501.CrossRefGoogle Scholar
  7. Chelkowski, J., 1991Cereal Grain Mycotoxins Fungi and Quality in Drying and Storage,Elsevier, Amsterdam.Google Scholar
  8. Christensen, C.M., 1974Storage of Cereal Grains and Their Products2nd Edition, American Association of Cereal-species Chemists, St. Paul, MN.Google Scholar
  9. Coker, R.D. 1998, Design of sampling plans for determination ofmycotoxins, in:Mycotoxins in Agriculture and Food SafetyK.K. Sinha and D. Bhatnagar, eds., Marcel Dekker, Inc., New York.Google Scholar
  10. Cotty, P.J., 1989, Virulence and cultural characteristics of twoAspergillus flavusstrains pathogenic in cotton,Phytopath.79:808.CrossRefGoogle Scholar
  11. Cony, P.J., 1990, Effect of atoxigenic strains ofAspergillus flavuson aflatoxin contamination of developing cottonseed,Pl. Dis.,74:233.CrossRefGoogle Scholar
  12. Cotty, P.J., 1997, Aflatoxin-producing potential of communities of Aspergillus section Flavi from cotton producing areas in the United StatesMycol. Res.6:698.CrossRefGoogle Scholar
  13. Cotty, P.J., and Bayman, P., 1993, Competitive exclusion of atoxigenic strain ofAspergillus flavusby an atoxigenic strain,Phytopath.83:1283.CrossRefGoogle Scholar
  14. Cotty, P.J., and Cardwell, K.F., 1999, Divergence of West African and North American communities of Aspergillus section FlaviAppl. Env. Microbiol65:2264.Google Scholar
  15. Cunnif, P., 1995, Official Methods of Analysis of AOAC International 16th Edition, AOAC International, Arlington, VA.Google Scholar
  16. Dimanche, P., 2000, Recent developments and trends in European regulations croncerning groundnut aflatoxin contamination: Consequences for groundnut production in Africa in Summary Proceedings of the Sixth ICRISAT Regional Groundnut Meeting for Western and Central Africa, Waliyar, F. and Ntare, B.R., eds, ICRISAT, Patancheru, IndiaGoogle Scholar
  17. Dorner, J. W., Cole, R.J., and Blankenship, P.D., 1992, Use of a biocompetitive agent to control preharvest aflatoxins in drought stressed peanutsJ. Food Protection55:888.Google Scholar
  18. Dowd, P.F., 1998, Involvement of arthropods in the establishment ofrnycotoxigenic fungi under field conditions in:Mycotoxins in Agriculture and Food SafetySinisa, K.K., and Bhatnagar, D., eds., Marcel Dekker, Inc., New York.Google Scholar
  19. Frisvad, J.C., 1995, Mycotoxins and mycotoxigenic fungi in storage in:Stored-Grain EcosystemsJayas, D.S., White, N.D.G, and Muir, W.E., eds., Marcel Dekker, Inc.,New York.Google Scholar
  20. Frisvad, J.C., and Samson, R.A., 1991, Mycotoxins produced by species of Penicillium and Aspergillus occurring in cereals, in:Cereal Grain Mycotoxins Fungi and Quality in Drying and StorageJ. Chelkowski, ed., Elsevier, Amsterdan.Google Scholar
  21. Gardner, D.E., McMeans, J.L., Brown, C.M., Bilbrey, R.M., and Parker, L.L., 1974, Geographic localization and lint fluorescence inAspergillus flavusinfected cottonseed.Phytopathology64:452–455.CrossRefGoogle Scholar
  22. Heenan, C.N., Shaw, K.S. and Pitt, J.I., 1998, Ochratoxin A production byAspergillus carbonariusandA. nigerand detection using coconut cream agar.J. Food Mycol.1:67.Google Scholar
  23. Hocking, A.D., 2001, Toxigenic Aspergillus species, in:Food Microbiology Fundamentals and FrontiersDoyle, M.P. Beuchat, L.R., and Montville, T.J., eds. ASM press, Baltimore.Google Scholar
  24. Horie, Y., 1995, Production of Ochratoxin A ofAspergillus carbonariusin Aspergillus section Nigri,Nippon Kingakukai Kaiho, 36:73.Google Scholar
  25. Horn, B.W., and Dorner, J.W., 1998, Soil populations of Aspergillus species from section Flavi along a transect through peanut-growing regions of the United StatesMycologia90:767.CrossRefGoogle Scholar
  26. Horn, B.W., and Dorner, J.W., 1999, Regional differences in production of aflatoxin Biand cyclopiazonic acid by soil isolates ofAspergillus flavusalong a transect within the United States,Appl. Env. Microbial, 65:1444.Google Scholar
  27. Jayas, D.S., White, N.D.G., and Muir, W.E., 1995, Stored-Grain Ecosystems, Marcel Dekker, Inc., New York.Google Scholar
  28. Klich, M.A., and Pitt, J.I., 1998, A Laboratory Guide to the Common Aspergillus Species and their Teleomorphs, CSIRO Division of Food Processing, North Ryde, Australia.Google Scholar
  29. Kurtzman, C.P., Smiley, M.J., Robnett, C.J. and Wicklow, D.T., 1986, DNA relatedness among wild and domesticated species in theAspergillus flavusgroup,Mycologia, 78:955.CrossRefGoogle Scholar
  30. Lacey, J., and Magon, N., 1991, Fungi in cereal grains: Their occurrence and water and temperature relationships, in:Cereal Grain Mycotoxins Fungi and Quality in Drying and Storage. Chelkowski, J. ed., Elsevier, Amsterdan.Google Scholar
  31. Manabe, J., and Tsuruta, O., 1991, Mycoflora and mycotoxins in stored rice grain in:Cereal Grain Mycotoxins Fungi and Quality in Drying and Storageed; Chelkowski, J., Elsevier, Amsterdan.Google Scholar
  32. Marsh, P.B., Simpson, M.E. and Filsinger, E.C., 1973Aspergillus flavusboll rot in the U.S. cotton belt in relation to high aqueous-extract p of fiber, and indicator of exposure to humid conditions. Pl. Dis. Reporter 57:664–668. Google Scholar
  33. Micheli, P.A., 1729, Nova Plantarum Genera Juxta Tournefortii Disposita. Florence.Google Scholar
  34. Murawski, D.A., 2000, FungiNat. Geographic198:58.Google Scholar
  35. Odum, T.V., Bigelow, D.M., Nelson, M.R., Howell, D.R., and Cotty, P.J., 1977, Spatial and temporal patterns ofAspergillus flavusstrain composition and propagule density in Yuma County, Arizona soils,Pl. Dis.81:911.Google Scholar
  36. Ominski, K.H., Marquardt, R.R., Sinha, R.N., and Abramson, D., 1994, Ecological aspects of growth and mycotoxin production by storage fungi in:Mycotoxins in Grain Compounds Other Than AflatoxinMiller, J.D., and Treuhohn, H.L., eds., Eagan Press, St. Paul, MN.Google Scholar
  37. Payne, G.A., 1998, Process of contamination by aflatoxin-producing fungi and their impact on crops in: Mycotoxins in Agriculture and Food Safety, Sinha, K.K. and Bhatnagar, D., eds., Marcel Dekker, Inc., New York.Google Scholar
  38. Pons, W.A., and Goldblatt, L.A., 1965, The determination of aflatoxins in cottonseed productsJ. Am. Oil Chem. Soc.42:471.CrossRefGoogle Scholar
  39. Raper, K.B., and Fennell, D.I., 1965, The Genus Aspergillus, Williams and Wilkins, Baltimore.Google Scholar
  40. Sauer, D.B., 1992, Storage of Cereal Grains and their Products4 thEdition, American Association of Cereal Chemists, St. Paul, MN.Google Scholar
  41. Sugimoto, T., Minamisawa, M., Takano, K., Furukawa, Y., and Tsuruta, O., 1976,Natural occurrence of ochratoxin and sterigmatocystin in moldy riceProc.Jpn. Assoc. Mycotoxicol3/4:3.Google Scholar
  42. Udoh, J.M., Cardwell, K.F., and Ikotun, T., 2000, Storage structures and aflatoxin content of maize in five agroecological zones of NigeriaJ. Stored Prod. Res36:187.CrossRefGoogle Scholar
  43. Urano, T., Trucksess, M.W., Beaver, R.W., Wilson, D.M., Dorner, J.W., and Dowell, F.E., 1992, Cooccurrence of cyclopiazonic acid and aflatoxins in corn and peanuts, JAOAC Int. 75:838. Google Scholar
  44. Vega, F.E., and Mercadier, G., 1998Florida Entomologist81:543.CrossRefGoogle Scholar
  45. Whitaker, T.B., and Park, D.L., 1994, Problems associated with accurately measuring aflatoxins in food and feeds: Errors associated with sampling, Sample preparation and analysis, in:The Toxicology of AflatoxinsEaton, D.L. and Groopman, J.D., eds., Academic Press, San Diego, CA.Google Scholar
  46. Wicklow, D.T., and Shotwell, O.L., 1983, Intrafungal distribution of aflatoxins among conidia and sclerotia ofAspergillus flavusandAspergillus parasiticus. Can. J. Microbiol.29:1.CrossRefGoogle Scholar
  47. Widstrom, N.W., 1992, Aflatoxin in developing maize: Interactions among involved biota and pertinent econiche factors, in:Handbook of Applied MycologyVolume 5: Mycotoxins in Ecological systems, Bhatnagar, D., Lillehoj, E.B., and Arora, D.K., eds., Marcel Dekker, Inc. New York.Google Scholar
  48. Will, M.E., Holbrook, C.C., and Wilson, D.M., 1994, Evaluation of field inoculation techiques for screening pea nut genotype: for reaction to preharvestA. flavusgroup infection and aflatoxin contamination,Peanut Sei., 21:122.CrossRefGoogle Scholar
  49. Williams, J.T. and Wilson D.M., 1999, Report on the Brazil nut aflatoxin problem is Bolivia, Upublished 24 page report to Chemonics and US AID. Wilson, D.M., 1995, Management ofmycotoxins in peanuts, in:Peanut Health ManagementMelouk, H.A., and Shokes, F.M., eds., APS Press, St. Paul, MN.Google Scholar
  50. Wilson, D.M., and Abramson, D., 1992, Mycotoxins, in:Storage of Cereal Grains and their ProductsSauer,D.B., ed., American Association of Cereal Chemists, St. Paul, MN.Google Scholar
  51. Wilson, D.M., and King, J.K., 1995, Production of aflatoxins Bi,B2,G, and G2 in pure and mixed cultures ofAspergillus parasiticus and Aspergillus flavus.Food Additives and Contaminants12:521.CrossRefGoogle Scholar
  52. Wilson, D.M., McMillian, W.W., and Widstrom, N.W., 1986, Use ofAspergillusflavusandA. parasiticuscolor mutants to study aflatoxins contamination in:BiodeteriorationVI, Liewellan, G., O=Rear, C., and Barry, S., eds., International Biodeterioration Society, London.Google Scholar
  53. Wilson, D.M., and Payne, G.A., 1994. Factors affectingAspergillus flavusgroup infection and aflatoxin contamination of crops in:The Toxicology of Aflatoxins, Eaton, D.L., and Groopman, J.D., eds., Academic Press, Inc. San Diego, CA.Google Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • David M. Wilson
    • 1
  • Wellington Mubatanhema
    • 1
  • Zeljko Jurjevic
    • 2
  1. 1.Department of Plant PathologyUniversity of GeorgiaTiftonUSA
  2. 2.Department of PhytopathologyUniversity of ZagrebZagrebCroatia

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