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Metabolomic Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

  • Dayan Goodenowe

Abstract

All organisms contain a finite set of genes. However, the expression of these genes under varying developmental and environmental conditions results in an almost infinite number of possible phenotypes. To properly study and understand an organism we need to know more than its genetic identity (i.e. its genome) and how its genome responds to developmental and environmental challenges. We need to know how these responses either benefit or harm the organism. To study properly the events leading to, and the effects resulting from, the expression of a gene we need to be able to characterize the corresponding phenotypic changes in an objective and quantifiable manner. Metabolomics is the science of phenotype analysis through the measurement of the small molecules (metabolites) that interact with, are used by, and are created by, the functionally active proteins of a biological system.

Keywords

Empirical Formula Accurate Mass Metabolomic Analysis Metabolite Identification Mastocytoma Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Espina JR, Shockcor JP, Herron WJ et al. Detection of in vivo biomarkers of phospholipidosis using NMR-based metabonomics approaches. Magn Reson Chem 28: 559–565 (2001).CrossRefGoogle Scholar
  2. Ivanova PT, Cerda B A, Horn DM et al. Electrospray ionization mass spectrometry analysis of changes in phospholipids in RBL-2H3 mastocytoma cells during degranulation. Proc Natl Acad Sci USA 98: 7152–7157 (2001).CrossRefGoogle Scholar
  3. Marshall AG, Grosshans PG. Fourier transform ion cyclotron resonance mass spectrometry; the teenage years. Anal Chem 63: 215A–229A (1991).Google Scholar
  4. Marshall AG. Milestones in Fourier transform ion cyclotron resonance mass spectrometry technique development. Int J Mass Spectrom 200: 331–356 (2000).CrossRefGoogle Scholar
  5. Marshall AG, Hendrickson CL. Fourier transform ion cyclotron resonance detection: principles and experimental configurations. Int J Mass Spectrom 215: 59–75 (2002).CrossRefGoogle Scholar
  6. Null AP, Muddiman DC. Perspectives on the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for short tandem repeat genotyping in the post-genome era. J Mass Spectrom 36: 589–606 (2001).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Dayan Goodenowe
    • 1
  1. 1.Phenomenome Discoveries Inc.SaskatoonCanada

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