Viral Entry into Host Cells pp 95-127

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 790)

Paramyxovirus Entry

  • Katharine N. Bossart
  • Deborah L. Fusco
  • Christopher C. Broder

Abstract

The family Paramyxoviridae consists of a group of large, enveloped, negative-sense, single-stranded RNA viruses and contains many important human and animal pathogens. Molecular and biochemical characterization over the past decade has revealed an extraordinary breadth of biological diversity among this family of viruses. Like all enveloped viruses, paramyxoviruses must fuse their membrane with that of a receptive host cell as a prerequisite for viral entry and infection. Unlike most other enveloped viruses, the vast majority of paramyxoviruses contain two distinctmembrane-anchored glycoproteins to mediate the attachment, membrane fusion and particle entry stages of host cell infection. The attachment glycoprotein is required for virion attachment and the fusion glycoprotein is directly involved in facilitating the merger of the viral and host cell membranes. Here we detail important functional, biochemical and structural features of the attachment and fusion glycoproteins from a variety of family members. Specifically, the three different classes of attachment glycoproteins are discussed, including receptor binding preference, their overall structure and fusion promotion activities. Recently solved atomic structures of certain attachment glycoproteins are summarized, and how they relate to both receptor binding and fusion mechanisms are described. For the fusion glycoprotein, specific structural domains and their proposed role in mediating membrane merger are illustrated, highlighting the important features of protease cleavage and associated tropism and virulence. The crystal structure solutions of both an uncleaved and a cleavage-activated metastable F are also described with emphasis on how small conformational changes can provide the necessary energy to mediate membrane fusion. Finally, the different proposed fusion models are reviewed, featuring recent experimental findings that speculate how the attachment and fusion glycoproteins work in concert to mediate virus entry.

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Copyright information

© Landes Bioscience and Springer Science+Business Media 2013

Authors and Affiliations

  • Katharine N. Bossart
    • 1
    • 2
  • Deborah L. Fusco
    • 3
  • Christopher C. Broder
    • 3
  1. 1.Department of MicrobiologyBoston University School of MedicineBostonUSA
  2. 2.National Emerging Infectious Diseases Laboratories InstituteBoston University School of MedicineBostonUSA
  3. 3.Department of Microbiology and ImmunologyUniformed Services UniversityBethesdaUSA

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