Interphase Chromosome-Specific Multicolor Banding
Interphase chromosome-specific multicolor banding (ICS-MCB) is a FISH-based technique for studying interphase chromosomes in their integrity at molecular resolution in single cells. To date, this is the only method providing for visualizing the whole chromosome and its specific regions together at any stage of the cell cycle. Offering such opportunity, ICS-MCB has repeatedly proven effective in molecular cytogenetic studies of interphase chromosomes (i.e., surveying genomic variations at the chromosomal level in different human tissues and characterizing interphase genome/chromosome organization and behavior). The intention of this chapter is to describe the basics of ICS-MCB procedure and its applications in different biomedical fields. The advantages of this technique allow speculations that it has to become a method of choice in somatic cell genetics as well as in cell and chromosome biology.
KeywordsInterphase Nucleus Molecular Resolution Chromosomal Mosaicism Interphase Chromosome Homologous Chromosome Pair
The authors are supported by DLR/BMBF (RUS 2011-2013) and RFBR grant 12-04-00215-а (Russian Federation, 2012–2014). The authors also acknowledge the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD, USA, for providing the brain tissue samples studied by ICS-MCB (Fig. 9.2). Dr. IY Iourov is supported by the Grant of the President of the Russian Federation MD-4401.2013.7.
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