The Discovery of GW Bodies
Human autoantibodies were a key to the discovery of GW bodies and their integral protein, GW182. This publication marks the tenth anniversary of the discovery of GW182. As it turns out, the discovery of GW182 was quite timely because it coincided with the elucidation of the RNA interference (RNAi) pathway, which is now known to have a major role in post-transcriptional gene regulation. Following our publication of the essential features of GW182 in 2002, laboratories from around the world began investigations that led to the elucidation of the role of GW182 in RNAi and other pathways of mRNA processing and degradation. This chapter reviews the discovery of GW182 and the description of GWB and some of the observations that followed that still remain to be elucidated.
The authors would like to acknowledge the efforts and expertise of numerous colleagues, collaborators, trainees and technologists.
Trainees: Dr. T. Eystathioy, Dr. Z. Yang, Dr. A. Jakymiw, Dr. S. Lian, Dr. S. Li, Dr. J. Moser, Dr. K. Pauley, Dr. L. Luft, Dr. R. Bhanji, K. Griffith, Dr. L. Stinton, Dr. K. Takeuchi.
Collaborators: Drs. D. Zochodne, M. Woods, J. Keene, S. Tenenbaum, B. Séraphin, N. Cougot, N. Kedersha, D. Bloch, L.E.C. Andrade, M. Mahler, M. Satoh, R. Mydlarski, L. Browder.
Technologists: John Hamel, Meifeng Zhang, Haiyan Hou, Maggie Lin, Jane Yang, Carol Peebles.
- Fritzler MJ, Hamel JC, Chan EKL (1992) Molecular characterization of an autoantigen of systemic lupus erythematosus. A unique an complete cDNA encoding a 95 kD protein of the Golgi complex. Arthritis Rheum 35:169Google Scholar
- Fritzler MJ, Stinton LM, Chan EKL (2007) Autoantibodies to cytoplasmic autoantigens in endosomes, exosomes and the Golgi complex. In: Conrad K, Chan EKL, Fritzler MJ, Sack U, Shoenfeld Y, Wiik A (eds) From etiopathogenesis to the prediction of autoimmune diseases: relevance of autoantibodies. Autoantigens, autoantibodies and autoimmunity. Proceedings 8th Dresden Symposium on Autoantibodies. Pabst Science Publishers, Lengerich, Germany, pp 194–209Google Scholar
- Lindstedt AD, Hauri HP (1993) Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Mol Biol Cell 4:679–693Google Scholar
- Luft LM (2005) Thesis Dissertation: Characterization of GWBs in Breast Cancer. University of Calgary.Google Scholar
- Nakamura RM, Peebles CL, Molden DP, Tan EM (1984) Advances in laboratory tests for autoantibodies to nuclear antigens in systemic rheumatic diseases. Lab Med 15:190–198Google Scholar
- Rattner JB, Wang T, Mack G, Valencia D, Fritzler MJ (1991) Identification of a novel human centromere protein, CENP-F. J Cell Biol 115(3):93aGoogle Scholar
- Rodriguez-Sanchez JL, Gelpi C, Juarez C, Hardin JA (1987) A new autoantibody in scleroderma that recognizes a 90-kDa component of the nucleolus-organizing region of chromatin. J Immunol 8:2579–2584Google Scholar
- Selak S, Schoenroth L, Senécal J-L, Fritzler MJ (1999) Early endosome antigen 1: an autoantigen associated with neurological diseases. J Invest Med 47:311–318Google Scholar