20 Years from NCX Purification and Cloning: Milestones

  • Debora A. Nicoll
  • Michela Ottolia
  • Joshua I. Goldhaber
  • Kenneth D. Philipson
Chapter

Abstract

The Na+/Ca2+ exchanger protein was first isolated from cardiac sarcolemma in 1988 and cloned in 1990. This allowed study of Na+/Ca2+ exchange at the molecular level to begin. I will review the story leading to the cloning of NCX and the research that resulted from this event. This will include structure-function studies such as determination of the numbers of transmembrane segments and topological arrangement. Information on ion transport sites has been gathered from site-directed mutagenesis. The regions involved in Ca2+ regulation have been identified, analyzed, and crystallized.

We have also generated genetically altered mice to study the role of NCX in the myocardium. Of special interest are mice with atrial- or ventricular-specific KO of NCX that reveal new information on the role of NCX in excitation-contraction coupling and in cardiac pacemaker activity.

Keywords

Na+/Ca2+ exchange Ca2+ transport Excitation-contraction coupling Ca2+ regulation Na+ transport Cardiac pacemaking 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Debora A. Nicoll
    • 1
  • Michela Ottolia
    • 1
  • Joshua I. Goldhaber
    • 2
  • Kenneth D. Philipson
    • 1
  1. 1.Departments of Physiology and Medicine and the Cardiovascular Research Laboratories MRL 3-645David Geffen School of Medicine at UCLALos AngelesUSA
  2. 2.Cedars-Sinai Heart InstituteLos AngelesUSA

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