Yersinia pestis Autoagglutination Is Mediated by HCP-Like Protein and Siderophore Yersiniachelin (Ych)

  • Olga PodladchikovaEmail author
  • Violetta Rykova
  • Uladzimir Antonenka
  • Alexander Rakin
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 954)


Autoagglutination (AA) is a virulence-associated trait typical of various pathogenic bacteria, including Yersinia pestis. In this study, we show that AA in Y. pestis is a complex phenomenon determined by the HCP-like protein of T6SS which is encoded by ypo0502 and by the siderophore Yersiniachelin (Ych) which is encoded by the ysu locus. Down-regulation of YPO0502 expression by antisense RNA abrogates AA in G8786 (Hms+) and EV76 (Hms) Y. pestis strains. The deletion of Ych biosynthetic genes ypo1530-1532 in EV76 though does not abolish AA results in its noticeable depression.


Yersinia Pestis Ammonium Sulfate Solution Kanamycin Resistance Cassette Hydroxamate Siderophore Siderophore Activity 
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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Olga Podladchikova
    • 1
    Email author
  • Violetta Rykova
    • 1
  • Uladzimir Antonenka
    • 2
  • Alexander Rakin
    • 2
  1. 1.Anti-plague Research InstituteRostov-on-DonRussia
  2. 2.Max von Pettenkofer Institut for Hygiene and Medical MicrobiologyLudwig Maximillians UniversityMunichGermany

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