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Yersinia pestis Autoagglutination Is Mediated by HCP-Like Protein and Siderophore Yersiniachelin (Ych)

  • Olga PodladchikovaEmail author
  • Violetta Rykova
  • Uladzimir Antonenka
  • Alexander Rakin
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 954)

Abstract

Autoagglutination (AA) is a virulence-associated trait typical of various pathogenic bacteria, including Yersinia pestis. In this study, we show that AA in Y. pestis is a complex phenomenon determined by the HCP-like protein of T6SS which is encoded by ypo0502 and by the siderophore Yersiniachelin (Ych) which is encoded by the ysu locus. Down-regulation of YPO0502 expression by antisense RNA abrogates AA in G8786 (Hms+) and EV76 (Hms) Y. pestis strains. The deletion of Ych biosynthetic genes ypo1530-1532 in EV76 though does not abolish AA results in its noticeable depression.

Keywords

Yersinia Pestis Ammonium Sulfate Solution Kanamycin Resistance Cassette Hydroxamate Siderophore Siderophore Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Olga Podladchikova
    • 1
    Email author
  • Violetta Rykova
    • 1
  • Uladzimir Antonenka
    • 2
  • Alexander Rakin
    • 2
  1. 1.Anti-plague Research InstituteRostov-on-DonRussia
  2. 2.Max von Pettenkofer Institut for Hygiene and Medical MicrobiologyLudwig Maximillians UniversityMunichGermany

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