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Autophagy Induction Does Not Protect Retina Against Apoptosis in Ischemia/Reperfusion Model

  • Nathalie Produit-ZengaffinenEmail author
  • Constantin J. Pournaras
  • Daniel F. Schorderet
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 801)

Abstract

The role played by autophagy after ischemia/reperfusion (I/R) in the retina remains unknown. Our study investigated whether ischemic injury in the retina, which causes an energy crisis, would induce autophagy. Retinal ischemia was induced by elevation of the intraocular pressure and modulation of autophagic markers was analyzed at the protein levels in an early and late phase of recovery. Following retinal ischemia an increase in LC3BII was first observed in the early phase of recovery but did not stay until the late phase of recovery. Post-ischemic induction of autophagy by intravitreal rapamycin administration did not provide protection against the lesion induced by the ischemic stress. On the contrary, an increase in the number of apoptotic cells was observed following I/R in the rapamycin treated retinas.

Keywords

Retinal ischemia Autophagy Apoptosis LC3 Rapamycin 

Abbreviations

I/R

Ischemia reperfusion

LC3

Microtubule associated protein light chain

CMA

Chaperone-mediated autophagy

Notes

Acknowledgement

The authors wish to thank Nicole Gilodi for expert technical help and Sue Houghton for reading the manuscript. This work was supported by a grant from the ProVisu Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Nathalie Produit-Zengaffinen
    • 1
    Email author
  • Constantin J. Pournaras
    • 2
  • Daniel F. Schorderet
    • 1
    • 3
    • 4
  1. 1.Institute for Research in OphthalmologySionSwitzerland
  2. 2.Department of NEUCLIDGeneva University HospitalsGenevaSwitzerland
  3. 3.Department of OphthalmologyUniversity of LausanneLausanneSwitzerland
  4. 4.Faculty of Life SciencesEcole polytechnique fédérale de LausanneLausanneSwitzerland

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