Autophagy Induction Does Not Protect Retina Against Apoptosis in Ischemia/Reperfusion Model
The role played by autophagy after ischemia/reperfusion (I/R) in the retina remains unknown. Our study investigated whether ischemic injury in the retina, which causes an energy crisis, would induce autophagy. Retinal ischemia was induced by elevation of the intraocular pressure and modulation of autophagic markers was analyzed at the protein levels in an early and late phase of recovery. Following retinal ischemia an increase in LC3BII was first observed in the early phase of recovery but did not stay until the late phase of recovery. Post-ischemic induction of autophagy by intravitreal rapamycin administration did not provide protection against the lesion induced by the ischemic stress. On the contrary, an increase in the number of apoptotic cells was observed following I/R in the rapamycin treated retinas.
KeywordsRetinal ischemia Autophagy Apoptosis LC3 Rapamycin
Microtubule associated protein light chain
The authors wish to thank Nicole Gilodi for expert technical help and Sue Houghton for reading the manuscript. This work was supported by a grant from the ProVisu Foundation.