Nanoceria and Thioredoxin Regulate a Common Antioxidative Gene Network in tubby Mice

  • Xue Cai
  • Junji Yodoi
  • Sudipta Seal
  • James F. McGinnis
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (volume 801)

Abstract

Oxidative stress is a node common to the causes and effects of various ocular diseases. We have shown that thioredoxin has neuroprotective effects on tubby photoreceptors. We also demonstrated that nanoceria (cerium oxide nanoparticles), which are direct antioxidants, have long-term effects on prevention of retinal degeneration in tubby mice. Here, using commercially available PCR array plates, we surveyed the regulation in expression of 89 oxidative stress-associated genes in the eyes of P12 tubby mice which are either intravitreally injected with nanoceria or in which the Trx gene is overexpressed. Our data demonstrate that nanoceria and Trx regulate the same group of genes associated with antioxidative stress and antioxidant defense.

Keywords

Tubby mouse Nanoceria Thioredoxin Oxidative stress and antioxidant defense PCR array 

Abbreviations

Trx

Thioredoxin

ASK1

Apoptosis signal kinase 1

MAPKs

Mitogen-activated protein kinases

Nanoceria

Cerium oxide nanoparticles

P7

Postnatal day 7

Wt

Wild type

IACUC

Institutional Animal Care and Use Committee

Nrf2

Nuclear factor erythroid 2-related factor

siRNA

Small interfering RNA

ROS

Reactive oxygen species

ICAM-1

Intercellular adhesion molecule 1

HMVEC

Human microvascular endothelial cells

Nxn

Nucleoredoxin

Cys

Cysteine residue

Wnt

Wingless/Intergration, Wg/Int

Dvl

Dishevelled

RPE

Retinal pigment epithelium

bZIP

Basic leucine zipper

ARACNE

Algorithm for the Reconstruction of Accurate Cellular Networks

CLR

Context Likelihood of Relatedness

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Copyright information

© Springer Science+Business Media, LLC 2014

Authors and Affiliations

  • Xue Cai
    • 1
  • Junji Yodoi
    • 2
  • Sudipta Seal
    • 3
  • James F. McGinnis
    • 1
    • 4
    • 5
    • 6
  1. 1.Department of Ophthalmology, Dean McGee Eye InstituteUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Department of Biological Responses, Institute for Virus ResearchKyoto UniversityKyotoJapan
  3. 3.Materials Science and Engineering, Advanced Materials Processing Analysis Center and Nanoscience Technology CenterUniversity of Central FloridaOrlandoUSA
  4. 4.Department of Cell BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  5. 5.Neuroscience CenterUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  6. 6.Department of Ophthalmology, Dean McGee Eye InstituteUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

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