Overview of Sequencing Technology Platforms
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The high-throughput DNA sequencing technologies are based on immobilization of the DNA samples onto a solid support, cyclic sequencing reactions using automated fluidics devices, and detection of molecular events by imaging. Featured sequencing technologies include: GS FLX by 454 Life Technologies/Roche, Genome Analyzer by Solexa/Illumina, SOLiD by Applied Biosystems, CGA Platform by Complete Genomics, and PacBio RS by Pacific Biosciences. In addition, emerging technologies are discussed.
KeywordsSequencing Library Rolling Circle Amplification Pacific Bioscience Sequencing Library Preparation Circular Consensus Sequencing
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