Overview of Sequencing Technology Platforms



The high-throughput DNA sequencing technologies are based on ­immobilization of the DNA samples onto a solid support, cyclic sequencing reactions using automated fluidics devices, and detection of molecular events by imaging. Featured sequencing technologies include: GS FLX by 454 Life Technologies/Roche, Genome Analyzer by Solexa/Illumina, SOLiD by Applied Biosystems, CGA Platform by Complete Genomics, and PacBio RS by Pacific Biosciences. In addition, emerging technologies are discussed.


Sequencing Library Rolling Circle Amplification Pacific Bioscience Sequencing Library Preparation Circular Consensus Sequencing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bentley, D. R. 2006. Whole-genome re-sequencing. Curr Opin Genet Dev 16 (6):545–552. doi:S0959-437X(06)00208-5 [pii]  10.1016/j.gde.2006.10.009.PubMedCrossRefGoogle Scholar
  2. Bentley, DR, S Balasubramanian, HP Swerdlow, GP Smith, J Milton, CG Brown, KP Hall et al. 2008. Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456:53–59.PubMedCrossRefGoogle Scholar
  3. Drmanac, R., A. B. Sparks, M. J. Callow, A. L. Halpern, N. L. Burns, B. G. Kermani, P. Carnevali et al. 2010. Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science 327 (5961):78–81. doi:1181498 [pii]  10.1126/science.1181498.PubMedCrossRefGoogle Scholar
  4. Eid, J, A Fehr, J Gray, K Luong, J Lyle, G Otto, P Peluso et al. 2009. Real-time DNA sequencing from single polymerase molecules. Science 323:133–138.PubMedCrossRefGoogle Scholar
  5. Flusberg, B. A., D. R. Webster, J. H. Lee, K. J. Travers, E. C. Olivares, T. A. Clark, J. Korlach, and S. W. Turner. 2010. Direct detection of DNA methylation during single-molecule, real-time sequencing. Nat Methods 7 (6):461–465. doi:nmeth.1459 [pii]  10.1038/nmeth.1459.PubMedCrossRefGoogle Scholar
  6. Korlach, J, A Bibillo, J Wegener, P Peluso, TT Pham, I Park, S Clark, GA Otto, and SW Turner. 2008. Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides. Nucleosides Nucleotides Nucleic Acids 27:1072–1083.PubMedCrossRefGoogle Scholar
  7. Korlach, J., P. J. Marks, R. L. Cicero, J. J. Gray, D. L. Murphy, D. B. Roitman, T. T. Pham, G. A. Otto, M. Foquet, and S. W. Turner. 2008. Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures. Proc Natl Acad Sci USA 105 (4):1176–1181.PubMedCrossRefGoogle Scholar
  8. Levene, M. J., J. Korlach, S. W. Turner, M. Foquet, H. G. Craighead, and W. W. Webb. 2003. ­Zero-mode waveguides for single-molecule analysis at high concentrations. Science 299 (5607):682–686.PubMedCrossRefGoogle Scholar
  9. Margulies, M., M. Egholm, W. E. Altman, S. Attiya, J. S. Bader, L. A. Bemben, J. Berka et al. 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437 (7057):376–380. doi:nature03959 [pii]  10.1038/nature03959.PubMedGoogle Scholar
  10. Shendure, J., G. J. Porreca, N. B. Reppas, X. Lin, J. P. McCutcheon, A. M. Rosenbaum, M. D. Wang, K. Zhang, R. D. Mitra, and G. M. Church. 2005. Accurate multiplex polony sequencing of an evolved bacterial genome. Science 309 (5741):1728–1732. doi:1117389 [pii]  10.1126/science.1117389.PubMedCrossRefGoogle Scholar
  11. Smith, A. M., L. E. Heisler, R. P. St Onge, E. Farias-Hesson, I. M. Wallace, J. Bodeau, A. N. Harris et al. 2010. Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples. Nucleic Acids Res 38 (13):e142.PubMedCrossRefGoogle Scholar
  12. Travers, K. J., C. S. Chin, D. R. Rank, J. S. Eid, and S. W. Turner. 2010. A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic Acids Res 38 (15):e159. doi:gkq543 [pii]  10.1093/nar/gkq543.PubMedCrossRefGoogle Scholar
  13. Valouev, A., J. Ichikawa, T. Tonthat, J. Stuart, S. Ranade, H. Peckham, K. Zeng et al. 2008. A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning. Genome Res 18 (7):1051–1063. doi:gr.076463.108 [pii]  10.1101/gr.076463.108. Google Scholar

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© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Division of Oncology, Department of Medicine, Stanford Genome Technology CenterStanford University School of Medicine, CCSRStanfordUSA

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