Overview of Sequencing Technology Platforms

  • Samuel Myllykangas
  • Jason Buenrostro
  • Hanlee P. Ji
Chapter

Abstract

The high-throughput DNA sequencing technologies are based on ­immobilization of the DNA samples onto a solid support, cyclic sequencing reactions using automated fluidics devices, and detection of molecular events by imaging. Featured sequencing technologies include: GS FLX by 454 Life Technologies/Roche, Genome Analyzer by Solexa/Illumina, SOLiD by Applied Biosystems, CGA Platform by Complete Genomics, and PacBio RS by Pacific Biosciences. In addition, emerging technologies are discussed.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Samuel Myllykangas
    • 1
  • Jason Buenrostro
    • 1
  • Hanlee P. Ji
    • 1
  1. 1.Division of Oncology, Department of Medicine, Stanford Genome Technology CenterStanford University School of Medicine, CCSRStanfordUSA

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