Finding a Novel Threadlike Structure on the Intra-abdominal Organ Surface of Small Pigs by Using In Vivo Trypan Blue Staining

  • Ayati M. Hossein
  • Tian Yu-Ying
  • Huang Tao
  • Zhang Yu-Qing
  • Che Yong-Zhe
  • Zhang Wei-Bo
Conference paper


Recently K.S. Soh and his colleagues in Seoul National University ­discovered novel threadlike structures named primo-vessels in organ surfaces, blood vessels, lymph vessels, and spinal cord in rabbits, rats, and mice. In this work, we tried to investigate whether the same structures can be found on the internal organ surface of small pigs. After being anesthetized with phenobarbital sodium and the abdomen opened, Trypan blue was applied to visualize the primo-vessels and primo-nodes on the internal organ surface of seven small pigs. Threadlike, movable, elastic structures could be observed on five small pigs. Morphological study on the obtained samples revealed that there are lots of rod-shaped nucleuses longitudinally along the structure. The structure is similar to the structures observed by K.S. Soh and his team in rat, rabbit, and mice. Further study is needed to investigate the function of these structures.


Trypan Blue Alcian Blue Alcian Blue Trypan Blue Solution Feulgen Reaction 
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This work was supported by a 973 project grant from the Ministry of Science and Technology of China (2010CB530507).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ayati M. Hossein
  • Tian Yu-Ying
  • Huang Tao
  • Zhang Yu-Qing
  • Che Yong-Zhe
  • Zhang Wei-Bo
    • 1
  1. 1.Institute of Acupuncture and MoxibustionChina Academy of Chinese Medical ScienceBeijingChina

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