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Development of the Putative Primo Vascular System Before the Formation of Vitelline Vessels in Chick Embryos

  • Seung-Yoon Lee
  • Byung-Cheon Lee
  • Kwang-Sup Soh
  • Gil-Ja JhonEmail author
Conference paper

Abstract

We found threadlike structures in the vitelline membrane and its surrounding dense albumen of a chick embryo by using the Trypan blue staining technique. The threadlike structure, a putative primo vessel, had DNA bodies as revealed by DAPI staining and was developed before the formation of blood vessels.

Keywords

Trypan Blue Chick Embryo Blood Vessel Formation Trypan Blue Staining Vitelline Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a “Systems Biology Infrastructure Establishment Grant” from the Gwangju Institute of Science and Technology. K. Soh thanks the Association of Korean Oriental Medicine for support.

References

  1. 1.
    Soh KS (2009) Bonghan circulatory system as an extension of acupuncture meridians. J Acupunct Meridian Stud 2(2):93–106PubMedCrossRefGoogle Scholar
  2. 2.
    Kim BH (1965) The Kyungrak system. J Jo Sun Med 108:1–38 [In Korean]Google Scholar
  3. 3.
    Pattern BM (1957) Early embryology of the chick, 4th edn. McGraw-Hill, New YorkGoogle Scholar
  4. 4.
    Lee BC, Kim KW, Soh KS (2009) Visualizing the network of Bonghan ducts in the omentum and peritoneum by using Trypan blue. J Acupunct Meridian Stud 2(1):66–70PubMedCrossRefGoogle Scholar
  5. 5.
    Lee BC, Bae KH, Jhon GJ et al (2009) Bonghan system as mesenchymal stem cell niches and pathways of macrophages in adipose tissues. J Acupunct Meridian Stud 2(1):79–82PubMedCrossRefGoogle Scholar
  6. 6.
    Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morph 88:49–92CrossRefGoogle Scholar
  7. 7.
    Pattern BM (1957) Early embryology of the chick, 4th edn. McGraw-Hill, New York, p 35Google Scholar
  8. 8.
    Kim BH (1965) Sanal theory. J Jo Sun Med 108:39–62 [In Korean]Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Seung-Yoon Lee
  • Byung-Cheon Lee
  • Kwang-Sup Soh
  • Gil-Ja Jhon
    • 1
    Email author
  1. 1.Department of Chemistry and Nano Science, College of Natural SciencesEwha Woman’s UniversitySeoulKorea

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