Development of the Putative Primo Vascular System Before the Formation of Vitelline Vessels in Chick Embryos

  • Seung-Yoon Lee
  • Byung-Cheon Lee
  • Kwang-Sup Soh
  • Gil-Ja JhonEmail author
Conference paper


We found threadlike structures in the vitelline membrane and its surrounding dense albumen of a chick embryo by using the Trypan blue staining technique. The threadlike structure, a putative primo vessel, had DNA bodies as revealed by DAPI staining and was developed before the formation of blood vessels.


Trypan Blue Chick Embryo Blood Vessel Formation Trypan Blue Staining Vitelline Membrane 
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This work was supported by a “Systems Biology Infrastructure Establishment Grant” from the Gwangju Institute of Science and Technology. K. Soh thanks the Association of Korean Oriental Medicine for support.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Seung-Yoon Lee
  • Byung-Cheon Lee
  • Kwang-Sup Soh
  • Gil-Ja Jhon
    • 1
    Email author
  1. 1.Department of Chemistry and Nano Science, College of Natural SciencesEwha Woman’s UniversitySeoulKorea

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